Prior acetaminophen consumption impacts the early adaptive cellular response of human skeletal muscle to resistance exercise

Andrew C.D. Lugos, Shivam H. Patel, Jordan C. Ormsby, Donald P. Curtis, Christopher Fry, Chad C. Carroll, Jared M. Dickinson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Resistance exercise (RE) is a powerful stimulus for skeletal muscle adaptation. Previous data demonstrate that cyclooxygenase (COX)-inhibiting drugs alter the cellular mechanisms regulating the adaptive response of skeletal muscle. The purpose of this study was to determine whether prior consumption of the COX inhibitor acetaminophen (APAP) alters the immediate adaptive cellular response in human skeletal muscle after RE. In a double-blinded, randomized, crossover design, healthy young men (n = 8, 25 ± 1 yr) performed two trials of unilateral knee extension RE (8 sets, 10 reps, 65% max strength). Subjects ingested either APAP (1,000 mg/6 h) or placebo (PLA) for 24 h before RE (final dose consumed immediately after RE). Muscle biopsies (vastus lateralis) were collected at rest and 1 h and 3 h after exercise. Mammalian target of rapamycin (mTOR) complex 1 signaling was assessed through immunoblot and immunohistochemistry, and mRNA expression of myogenic genes was examined via RTqPCR. At 1 h p-rpS6Ser240/244 was increased in both groups but to a greater extent in PLA. At 3 h p-S6K1Thr389 was elevated only in PLA. Furthermore, localization of mTOR to the lysosome (LAMP2) in myosin heavy chain (MHC) II fibers increased 3 h after exercise only in PLA. mTOR-LAMP2 colocalization in MHC I fibers was greater in PLA vs. APAP 1 h after exercise. Myostatin mRNA expression was reduced 1 h after exercise only in PLA. MYF6 mRNA expression was increased 1 h and 3 h after exercise only in APAP. APAP consumption appears to alter the early adaptive cellular response of skeletal muscle to RE. These findings further highlight the mechanisms through which COX-inhibiting drugs impact the adaptive response of skeletal muscle to exercise. All rights reserved.

Original languageEnglish (US)
Pages (from-to)1012-1024
Number of pages13
JournalJournal of Applied Physiology
Volume124
Issue number4
DOIs
StatePublished - Apr 1 2018

Fingerprint

Acetaminophen
Skeletal Muscle
Exercise
Placebos
Myosin Heavy Chains
Sirolimus
Prostaglandin-Endoperoxide Synthases
Messenger RNA
Myostatin
Cyclooxygenase Inhibitors
Quadriceps Muscle
Lysosomes
Pharmaceutical Preparations
Cross-Over Studies
Knee
Immunohistochemistry
Biopsy
Gene Expression
Muscles

Keywords

  • COX inhibitor
  • Exercise
  • M TORC1

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Lugos, A. C. D., Patel, S. H., Ormsby, J. C., Curtis, D. P., Fry, C., Carroll, C. C., & Dickinson, J. M. (2018). Prior acetaminophen consumption impacts the early adaptive cellular response of human skeletal muscle to resistance exercise. Journal of Applied Physiology, 124(4), 1012-1024. https://doi.org/10.1152/japplphysiol.00922.2017

Prior acetaminophen consumption impacts the early adaptive cellular response of human skeletal muscle to resistance exercise. / Lugos, Andrew C.D.; Patel, Shivam H.; Ormsby, Jordan C.; Curtis, Donald P.; Fry, Christopher; Carroll, Chad C.; Dickinson, Jared M.

In: Journal of Applied Physiology, Vol. 124, No. 4, 01.04.2018, p. 1012-1024.

Research output: Contribution to journalArticle

Lugos, Andrew C.D. ; Patel, Shivam H. ; Ormsby, Jordan C. ; Curtis, Donald P. ; Fry, Christopher ; Carroll, Chad C. ; Dickinson, Jared M. / Prior acetaminophen consumption impacts the early adaptive cellular response of human skeletal muscle to resistance exercise. In: Journal of Applied Physiology. 2018 ; Vol. 124, No. 4. pp. 1012-1024.
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