Prior exposure to uninfected mosquitoes enhances mortality in naturally-transmitted West Nile virus infection

Bradley S. Schneider, Charles E. McGee, Jeffrey M. Jordan, Heather Stevenson-Lerner, Lynn Soong, Stephen Higgs

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background. The global emergence of West Nile virus (WNV) has highlighted the importance of mosquito-borne viruses. These are inoculated in vector saliva into the vertebrate skin and circulatory system, Arthropod-borne (arbo)viruses such as WNV are transmitted to vertebrates as an infectious mosquito probes the skin for blood, depositing the virus and saliva into the skin and circulation. Growing evidence has demonstrated that arthropod, and recently mosquito, saliva can have a profound effect on pathogen transmission efficiency, pathogenesis, and disease course. A potentially important aspect of natural infections that has been ignored is that in nature vertebrates are typically exposed to the feeding of uninfected mosquitoes prior to the mosquito that transmits WNV. The possibility that pre-exposure to mosquito saliva might modulate WNV infection was explored. Principal Findings. Here we report that sensitization to mosquito saliva exacerbates viral infection, Prior exposure of mice to mosquito feeding resulted in increased mortality following WNV infection. This aggravated disease course was associated with enhanced early viral replication, increased interleukin-10 expression, and elevated influx of WNV-susceptible cell types to the inoculation site. This exacerbated disease course was mimicked by passive transfer of mosquito-sensitized serum. Significance. This is the first report that sensitization to arthropod saliva can exacerbate arthropod-borne infection, contrary to previous studies with parasite and bacteria infections. This research suggests that in addition to the seroreactivity of the host to virus, it is important to take into account the immune response to vector feeding.

Original languageEnglish (US)
Article numbere1171
JournalPLoS One
Volume2
Issue number11
DOIs
StatePublished - Nov 14 2007

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West Nile virus
Virus Diseases
Culicidae
Viruses
saliva
Saliva
Mortality
infection
Arthropods
disease course
skin (animal)
Vertebrates
arthropods
Skin
vertebrates
viruses
Arboviruses
circulatory system
arboviruses
Parasitic Diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Prior exposure to uninfected mosquitoes enhances mortality in naturally-transmitted West Nile virus infection. / Schneider, Bradley S.; McGee, Charles E.; Jordan, Jeffrey M.; Stevenson-Lerner, Heather; Soong, Lynn; Higgs, Stephen.

In: PLoS One, Vol. 2, No. 11, e1171, 14.11.2007.

Research output: Contribution to journalArticle

Schneider, Bradley S. ; McGee, Charles E. ; Jordan, Jeffrey M. ; Stevenson-Lerner, Heather ; Soong, Lynn ; Higgs, Stephen. / Prior exposure to uninfected mosquitoes enhances mortality in naturally-transmitted West Nile virus infection. In: PLoS One. 2007 ; Vol. 2, No. 11.
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