Abstract
Time-resolved fluorescence Stokes shifts (TRFSS) of 4′,6-diamidino-2- phenylindole (DAPI) inside the minor groove of DNA are measured in the presence of three different monovalent counterions: sodium (Na +), rubidium (Rb +), and tetrabutylammonium (TBA +). Fluorescence up-conversion and time-correlated single photon counting are combined to obtain the time-resolved emission spectra (TRES) of DAPI in DNA from 100 fs to 10 ns. Time-resolved Stokes shift data suggest that groove-bound DAPI can not sense the counterion dynamics because the ions are displaced by DAPI far from the probe-site. However, when these results are compared to the earlier base-stacked coumarin data, the same ions are found to affect the nanosecond dynamics significantly. This suggests that the ions come close to the probe-site, such that they can affect the dynamics when measured by base-stacked coumarin. These results support previous molecular dynamics (MD) simulation data of groove-bound and base-stacked probes inside DNA.
Original language | English (US) |
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Pages (from-to) | 2621-2626 |
Number of pages | 6 |
Journal | Journal of Physical Chemistry Letters |
Volume | 3 |
Issue number | 18 |
DOIs | |
State | Published - Sep 20 2012 |
Externally published | Yes |
Keywords
- Biophysical Chemistry and Biomolecules
ASJC Scopus subject areas
- Materials Science(all)
- Physical and Theoretical Chemistry