Probing the Mechanism of CRP Activation by Site-Directed Mutagenesis: The Role of Serine 128 in the Allosteric Pathway of cAMP Receptor Protein Activation

Keyphrases

• Site-directed mutagenesis 100%
• CAMP Receptor Protein 100%
• Protein Activation 100%
• Allosteric Mechanism 100%
• DNA Binding 18%
• Ligand Binding 18%
• Protein Subunit 18%
• Subunit Interaction 18%
• Protein Stability 18%
• DNA Affinity 9%
• Activation Mechanism 9%
• Escherichia Coli 9%
• Conformational Change 9%
• Ligand-binding Domain 9%
• Domain-domain Interaction 9%
• Molecular Events 9%
• Specific mutation 9%
• Point mutation 9%
• Biophysical Properties 9%
• Cyclic GMP 9%
• Biochemical Properties 9%
• Secondary Structure 9%
• Sedimentation Technique 9%
• Binding Affinity 9%
• Proteins Control 9%
• Spectroscopic Techniques 9%
• Subunit Association 9%
• Bound Form 9%
• Negative Cooperativity 9%
• Proteolytic Digestion 9%
• Interfacial Interaction 9%
• Circular Dichroism 9%
• Partial Reaction 9%
• Specific DNA Sequence 9%
• Protein Dimerization 9%
• Spectroscopic Probe 9%
• Catabolites 9%
• Quantitative Responses 9%
• Sensitivity Genes 9%
• Interaction Stability 9%
• Binding Reaction 9%
• Second Ligand 9%

Biochemistry, Genetics and Molecular Biology

• Site-Directed Mutagenesis 100%
• Cyclic Adenosine Monophosphate 100%
• Serine 100%
• Wild Type 16%
• Energy Transfer 11%
• Ligand Binding 11%
• Protein Stability 11%
• Binding Affinity 5%
• DNA Binding 5%
• Conformational Change 5%
• Escherichia coli 5%
• Protein Catabolism 5%
• DNA Sequence 5%
• Point Mutation 5%
• Protein Secondary Structure 5%
• Spectroscopy 5%
• Circular Dichroism 5%
• Cooperation 5%
• Ligand Binding Domain 5%
• Protein Dimer 5%