Processing of neuropeptide Y, galanin, and somatostatin in the cerebrospinal fluid of patients with Alzheimer's disease and frontotemporal dementia.

C. L. Nilsson, A. Brinkmalm, L. Minthon, K. Blennow, R. Ekman

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Alzheimer's disease (AD) and frontotemporal dementia (FTD) are two prevalent neurodegenerative disorders for which the causes are unknown, except in rare familial cases. Several changes in neuropeptide levels as measured by radioimmunoassay (RIA) have been observed in these illnesses. Somatostatin (SOM) levels in cerebrospinal fluid (CSF) are consistently decreased in AD and FTD. Neuropeptide Y (NPY) levels are decreased in AD, but normal in FTD. Galanin (GAL) levels increase with the duration of illness in AD patients. The majority of studies of neuropeptides in CSF have not been verified by HPLC. The observed decrease in a neuropeptide level as measured by RIA may therefore reflect an altered synthesis or extracellular processing, resulting in neuropeptide fragments that may or may not be detected by RIA. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-MS) has been shown to be a powerful technique in the analysis of biological materials without any pre-treatment, by detecting peptides and proteins at a specific mass-to-charge (m/z) ratio. We studied the processing of the neuropeptides NPY, NPY, SOM and GAL in the cerebrospinal fluid of patients with AD (n = 3), FTD (n = 3) and controls (n = 2) using MALDI-MS. We found that considerable inter-individual variability exists in the rate of neuropeptide metabolism in CSF, as well as the number of peptide fragments formed. Certain patients showed differences in the processing of specific neuropeptides, relative to other patients and controls. This analysis of the metabolic processing of neuropeptides in CSF yielded a large amount of data for each individual studied. Further studies are required to determine the changes in neuropeptide processing that can be associated with AD and FTD. With further investigations using MALDI-MS analysis, it may be possible to identify a neuropeptide fragment or processing enzyme that can be correlated to these disease states.

Original languageEnglish (US)
Pages (from-to)2105-2112
Number of pages8
JournalPeptides
Volume22
Issue number12
StatePublished - Dec 2001
Externally publishedYes

Fingerprint

Cerebrospinal fluid
Galanin
Frontotemporal Dementia
Neuropeptide Y
Somatostatin
Neuropeptides
Cerebrospinal Fluid
Alzheimer Disease
Processing
Radioimmunoassay
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Peptide Fragments
Metabolism
Neurodegenerative Diseases
Biological materials
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Processing of neuropeptide Y, galanin, and somatostatin in the cerebrospinal fluid of patients with Alzheimer's disease and frontotemporal dementia. / Nilsson, C. L.; Brinkmalm, A.; Minthon, L.; Blennow, K.; Ekman, R.

In: Peptides, Vol. 22, No. 12, 12.2001, p. 2105-2112.

Research output: Contribution to journalArticle

Nilsson, CL, Brinkmalm, A, Minthon, L, Blennow, K & Ekman, R 2001, 'Processing of neuropeptide Y, galanin, and somatostatin in the cerebrospinal fluid of patients with Alzheimer's disease and frontotemporal dementia.', Peptides, vol. 22, no. 12, pp. 2105-2112.
Nilsson, C. L. ; Brinkmalm, A. ; Minthon, L. ; Blennow, K. ; Ekman, R. / Processing of neuropeptide Y, galanin, and somatostatin in the cerebrospinal fluid of patients with Alzheimer's disease and frontotemporal dementia. In: Peptides. 2001 ; Vol. 22, No. 12. pp. 2105-2112.
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