Production and characterization of monoclonal antibodies to Rickettsia rickettsii

J. V. Lange, David Walker

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Five mouse ascitic fluids (MAFs) containing monoclonal antibody to Rickettsia rickettsii were produced from three original fusions by murine hybridoma technology. The five MAFs were fractionated and purified; each contained monoclonal antibody of the immunoglobulin G2a subclass. Each monoclonal antibody-containing MAF was titrated by indirect immunofluorescence against three R. rickettsii isolates from humans and four other spotted fever group rickettsiae. Each MAF was also titrated in the complement fixation, latex agglutination, microagglutination, and indirect hemagglutination tests. Two of the MAFs were examined for their ability to prevent fever and rickettsemia in susceptible guinea pigs after a 1:100 dilution of each was mixed with viable R. rickettsii, and all five MAFs were titrated in the mouse toxicity phenomenon assay. All MAFs had high indirect immunofluorescence titers to the three strains of R. rickettsii (1:200,000 to 1:800,000), reduced indirect immunofluorescence titers to R. montana, and were nonreactive with R. akari, R. sibirica, and R. conorii. Each MAF was able to fix complement in the presence of spotted fever group antigen reagent and agglutinate a suspension of purified R. rickettsii, and each was negative in both the latex agglutination and the indirect hemagglutination tests. The two MAFs which were tested proved to be capable of preventing rickettsemia and death in guinea pigs, and each MAF was able to prevent death in mice at dilutions ranging from 1:40 to 1:80.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalInfection and Immunity
Volume46
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

Rickettsia rickettsii
Ascitic Fluid
Monoclonal Antibodies
Indirect Fluorescent Antibody Technique
Hemagglutination Tests
Fever
Agglutination
Latex
Guinea Pigs
Rickettsia
Hybridomas

ASJC Scopus subject areas

  • Immunology

Cite this

Production and characterization of monoclonal antibodies to Rickettsia rickettsii. / Lange, J. V.; Walker, David.

In: Infection and Immunity, Vol. 46, No. 2, 1984, p. 289-294.

Research output: Contribution to journalArticle

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