Production of antigens for ELISA

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Immunologic assays such as ELISA allow detection of either virus antigens or the host’s immune response to antigens associated with prior infections and offer a powerful means to approach understanding the epidemiology and epizootiology of these agents. However, the success of these assays is highly dependent on the production of high-quality materials both to establish the assays (i.e., antigen target for antibody) and to serve as controls for establishing assay parameters and sensitivity. Here we describe methods for preparing ebolavirus antigens suitable for use as either reagents or controls in a variety of ELISA formats. Considering work with filoviruses is typically restricted to maximum containment laboratories; thus, we also provide methods for inactivating and safety testing of these antigens for safe use in properly established field laboratories.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages353-362
Number of pages10
Volume1628
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1628
ISSN (Print)1064-3745

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Keywords

  • Antigen production
  • Detection
  • Diagnostics
  • Ebola
  • ELISA
  • Filovirus
  • Surveillance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Cross, R., & Ksiazek, T. (2017). Production of antigens for ELISA. In Methods in Molecular Biology (Vol. 1628, pp. 353-362). (Methods in Molecular Biology; Vol. 1628). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7116-9_28