Production of dimethyl triselenide and dimethyl diselenenyl sulfide in the headspace of metalloid-resistant Bacillus species grown in the presence of selenium oxyanions

Radhika Burra, Gonzalo A. Pradenas, Rebecca A. Montes, Claudio C. Vásquez, Thomas G. Chasteen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A Bacillus species harvested from the environment is metalloid resistant and, when grown anaerobically in complex growth medium and amended with the selenium oxyanion selenate, selenite, or selenocyanate, produces volatile organoselenium compounds in bacterial culture headspace. Two novel compounds so far undetected in bacterial culture headspace, CH3Se2SCH3 and CH3SeSeSeCH3, are produced and can be detected using solid-phase microextraction and gas chromatography with either fluorine-induced chemiluminescence or mass spectrometric detection. Differences in the electron impact fragmentation pattern of the mixed sulfur/selenide compounds allow the tentative differentiation between the symmetric and asymmetric isomers in this bacterium's headspace in favor of the asymmetric CH3SeSeSCH3 isomer.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalAnalytical Biochemistry
Volume396
Issue number2
DOIs
StatePublished - Jan 15 2010
Externally publishedYes

Fingerprint

Organoselenium Compounds
Metalloids
Selenic Acid
Selenious Acid
Sulfur Compounds
Solid Phase Microextraction
Fluorine
Bacilli
Selenium
Luminescence
Isomers
Gas Chromatography
Bacillus
Electrons
Bacteria
Chemiluminescence
Sulfur compounds
Growth
Gas chromatography
dimethyl sulfide

Keywords

  • Bioremediation
  • Organoselenium
  • Polyselenides
  • Polysulfides
  • Volatilization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Production of dimethyl triselenide and dimethyl diselenenyl sulfide in the headspace of metalloid-resistant Bacillus species grown in the presence of selenium oxyanions. / Burra, Radhika; Pradenas, Gonzalo A.; Montes, Rebecca A.; Vásquez, Claudio C.; Chasteen, Thomas G.

In: Analytical Biochemistry, Vol. 396, No. 2, 15.01.2010, p. 217-222.

Research output: Contribution to journalArticle

Burra, Radhika ; Pradenas, Gonzalo A. ; Montes, Rebecca A. ; Vásquez, Claudio C. ; Chasteen, Thomas G. / Production of dimethyl triselenide and dimethyl diselenenyl sulfide in the headspace of metalloid-resistant Bacillus species grown in the presence of selenium oxyanions. In: Analytical Biochemistry. 2010 ; Vol. 396, No. 2. pp. 217-222.
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