Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor

Yazad Irani, Helen M. Brereton, Ronald Tilton, Douglas J. Coster, Keryn A. Williams

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) plays a major role in the development of aberrant neovascularization in ocular diseases such as diabetic retinopathy and age-related macular degeneration (ARMD), and is an important therapeutic target for these diseases. Monoclonal antibodies specific for VEGF are in clinical use for some patients with ARMD, delivered by intraocular injection. We have shown previously that single chain antibody fragments (scFv) penetrate into the eye when applied topically to the ocular surface. Here we describe the production of a scFv from a monoclonal antibody specific for human VEGF and demonstrate its ability to decrease proliferation of human umbilical vein endothelial cells in culture. A suitably formulated anti-VEGF scFv may have potential as a less invasive topical treatment for potentially blinding neovascular diseases of the eye.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalHybridoma
Volume28
Issue number3
DOIs
StatePublished - Jun 1 2009

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Single-Chain Antibodies
Immunoglobulin Fragments
Hybridomas
Vascular Endothelial Growth Factor A
Eye Diseases
Macular Degeneration
Monoclonal Antibodies
Intraocular Injections
Human Umbilical Vein Endothelial Cells
Diabetic Retinopathy
Cell Culture Techniques
Therapeutics
human VEGFA protein

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor. / Irani, Yazad; Brereton, Helen M.; Tilton, Ronald; Coster, Douglas J.; Williams, Keryn A.

In: Hybridoma, Vol. 28, No. 3, 01.06.2009, p. 205-209.

Research output: Contribution to journalArticle

Irani, Yazad ; Brereton, Helen M. ; Tilton, Ronald ; Coster, Douglas J. ; Williams, Keryn A. / Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor. In: Hybridoma. 2009 ; Vol. 28, No. 3. pp. 205-209.
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