Profile of human T cell response to leishmanial antigens. Analysis by immunoblotting

Peter Melby, F. A. Neva, D. L. Sacks

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Control and resolution of leishmanial infection depends primarily on T cell-mediated immune mechanisms. The nature of the leishmanial antigens involved in eliciting T cell immunity is unknown. We have examined the pattern of peripheral blood lymphocyte responses in patients with active, healed, or subclinical leishmanial infection to fractionated leishmanial antigens using a T cell immunoblotting method in which nitrocellulose-bound leishmanial antigens, are incorporated into lymphocyte cultures. The proliferative and IFN-γ responses of cells from patients with healed mucosal or cutaneous leishmaniasis were remarkably heterogeneous and occurred to as many as 50-70 distinct antigens. In contrast, responses from subjects with active, nonhealing, diffuse cutaneous leishamniasis were either absent or present to only a small number of antigens. Control and resolution of leishmaniasis, and resistance to reinfection, is therefore associated with a T cell response to a large and diverse pool of parasite antigens. The method of T cell immunoblotting appears to offer a powerful, rapid, and relatively simple approach to the identification of antigens involved in eliciting a T cell response in human leishmaniasis.

Original languageEnglish (US)
Pages (from-to)1868-1875
Number of pages8
JournalJournal of Clinical Investigation
Volume83
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Immunoblotting
T-Lymphocytes
Antigens
Leishmaniasis
Lymphocytes
Cutaneous Leishmaniasis
Asymptomatic Infections
Collodion
Immunity
Parasites
Skin
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Profile of human T cell response to leishmanial antigens. Analysis by immunoblotting. / Melby, Peter; Neva, F. A.; Sacks, D. L.

In: Journal of Clinical Investigation, Vol. 83, No. 6, 1989, p. 1868-1875.

Research output: Contribution to journalArticle

@article{033d33c4e3524a9aae46e33bc1d24027,
title = "Profile of human T cell response to leishmanial antigens. Analysis by immunoblotting",
abstract = "Control and resolution of leishmanial infection depends primarily on T cell-mediated immune mechanisms. The nature of the leishmanial antigens involved in eliciting T cell immunity is unknown. We have examined the pattern of peripheral blood lymphocyte responses in patients with active, healed, or subclinical leishmanial infection to fractionated leishmanial antigens using a T cell immunoblotting method in which nitrocellulose-bound leishmanial antigens, are incorporated into lymphocyte cultures. The proliferative and IFN-γ responses of cells from patients with healed mucosal or cutaneous leishmaniasis were remarkably heterogeneous and occurred to as many as 50-70 distinct antigens. In contrast, responses from subjects with active, nonhealing, diffuse cutaneous leishamniasis were either absent or present to only a small number of antigens. Control and resolution of leishmaniasis, and resistance to reinfection, is therefore associated with a T cell response to a large and diverse pool of parasite antigens. The method of T cell immunoblotting appears to offer a powerful, rapid, and relatively simple approach to the identification of antigens involved in eliciting a T cell response in human leishmaniasis.",
author = "Peter Melby and Neva, {F. A.} and Sacks, {D. L.}",
year = "1989",
language = "English (US)",
volume = "83",
pages = "1868--1875",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "6",

}

TY - JOUR

T1 - Profile of human T cell response to leishmanial antigens. Analysis by immunoblotting

AU - Melby, Peter

AU - Neva, F. A.

AU - Sacks, D. L.

PY - 1989

Y1 - 1989

N2 - Control and resolution of leishmanial infection depends primarily on T cell-mediated immune mechanisms. The nature of the leishmanial antigens involved in eliciting T cell immunity is unknown. We have examined the pattern of peripheral blood lymphocyte responses in patients with active, healed, or subclinical leishmanial infection to fractionated leishmanial antigens using a T cell immunoblotting method in which nitrocellulose-bound leishmanial antigens, are incorporated into lymphocyte cultures. The proliferative and IFN-γ responses of cells from patients with healed mucosal or cutaneous leishmaniasis were remarkably heterogeneous and occurred to as many as 50-70 distinct antigens. In contrast, responses from subjects with active, nonhealing, diffuse cutaneous leishamniasis were either absent or present to only a small number of antigens. Control and resolution of leishmaniasis, and resistance to reinfection, is therefore associated with a T cell response to a large and diverse pool of parasite antigens. The method of T cell immunoblotting appears to offer a powerful, rapid, and relatively simple approach to the identification of antigens involved in eliciting a T cell response in human leishmaniasis.

AB - Control and resolution of leishmanial infection depends primarily on T cell-mediated immune mechanisms. The nature of the leishmanial antigens involved in eliciting T cell immunity is unknown. We have examined the pattern of peripheral blood lymphocyte responses in patients with active, healed, or subclinical leishmanial infection to fractionated leishmanial antigens using a T cell immunoblotting method in which nitrocellulose-bound leishmanial antigens, are incorporated into lymphocyte cultures. The proliferative and IFN-γ responses of cells from patients with healed mucosal or cutaneous leishmaniasis were remarkably heterogeneous and occurred to as many as 50-70 distinct antigens. In contrast, responses from subjects with active, nonhealing, diffuse cutaneous leishamniasis were either absent or present to only a small number of antigens. Control and resolution of leishmaniasis, and resistance to reinfection, is therefore associated with a T cell response to a large and diverse pool of parasite antigens. The method of T cell immunoblotting appears to offer a powerful, rapid, and relatively simple approach to the identification of antigens involved in eliciting a T cell response in human leishmaniasis.

UR - http://www.scopus.com/inward/record.url?scp=0024313394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024313394&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 1868

EP - 1875

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 6

ER -