Profiling neurotransmitter receptor expression in the Ambystoma mexicanum brain

Jorge Mauricio Reyes-Ruiz, Agenor Limon-Ruiz, Matthew J. Korn, Paul A. Nakamura, Nicole J. Shirkey, Jamie K. Wong, Ricardo Miledi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ability to regenerate limbs and central nervous system (CNS) is unique to few vertebrates, most notably the axolotl (Ambystoma sp.). However, despite the fact the neurotransmitter receptors are involved in axonal regeneration, little is known regarding its expression profile. In this project, RT-PCR and qPCR were performed to gain insight into the neurotransmitter receptors present in Ambystoma. Its functional ability was studied by expressing axolotl receptors in Xenopus laevis oocytes by either injection of mRNA or by direct microtransplantation of brain membranes. Oocytes injected with axolotl mRNA expressed ionotropic receptors activated by GABA, aspartate. +. glycine and kainate, as well as metabotropic receptors activated by acetylcholine and glutamate. Interestingly, we did not see responses following the application of serotonin. Membranes from the axolotl brain were efficiently microtransplanted into Xenopus oocytes and two types of native GABA receptors that differed in the temporal course of their responses and affinities to GABA were observed. Results of this study are necessary for further characterization of axolotl neurotransmitter receptors and may be useful for guiding experiments aimed at understanding activity-dependant limb and CNS regeneration.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalNeuroscience Letters
Volume538
DOIs
StatePublished - Mar 22 2013
Externally publishedYes

Fingerprint

Ambystoma mexicanum
Neurotransmitter Receptor
Ambystoma
Brain
Oocytes
GABA Receptors
Regeneration
Extremities
Central Nervous System
Messenger RNA
Membranes
Kainic Acid
Xenopus laevis
Glutamate Receptors
Cholinergic Receptors
Xenopus
Aspartic Acid
Glycine
gamma-Aminobutyric Acid
Vertebrates

Keywords

  • Axolotl
  • CNS regeneration
  • Electrophysiology
  • Microtransplantation, QPCR
  • Xenopus oocytes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reyes-Ruiz, J. M., Limon-Ruiz, A., Korn, M. J., Nakamura, P. A., Shirkey, N. J., Wong, J. K., & Miledi, R. (2013). Profiling neurotransmitter receptor expression in the Ambystoma mexicanum brain. Neuroscience Letters, 538, 32-37. https://doi.org/10.1016/j.neulet.2013.01.015

Profiling neurotransmitter receptor expression in the Ambystoma mexicanum brain. / Reyes-Ruiz, Jorge Mauricio; Limon-Ruiz, Agenor; Korn, Matthew J.; Nakamura, Paul A.; Shirkey, Nicole J.; Wong, Jamie K.; Miledi, Ricardo.

In: Neuroscience Letters, Vol. 538, 22.03.2013, p. 32-37.

Research output: Contribution to journalArticle

Reyes-Ruiz, JM, Limon-Ruiz, A, Korn, MJ, Nakamura, PA, Shirkey, NJ, Wong, JK & Miledi, R 2013, 'Profiling neurotransmitter receptor expression in the Ambystoma mexicanum brain', Neuroscience Letters, vol. 538, pp. 32-37. https://doi.org/10.1016/j.neulet.2013.01.015
Reyes-Ruiz, Jorge Mauricio ; Limon-Ruiz, Agenor ; Korn, Matthew J. ; Nakamura, Paul A. ; Shirkey, Nicole J. ; Wong, Jamie K. ; Miledi, Ricardo. / Profiling neurotransmitter receptor expression in the Ambystoma mexicanum brain. In: Neuroscience Letters. 2013 ; Vol. 538. pp. 32-37.
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