Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin

Vidyavathi Udupi, Heung Man Lee, Alexander Kurosky, George H. Greeley

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)123-127
Number of pages5
JournalRegulatory Peptides
Volume83
Issue number2-3
DOIs
StatePublished - Sep 15 1999

Keywords

  • Antisense
  • Endoprotease
  • Peptide
  • Processing

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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