Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin

Vidyavathi Udupi, Heung Man Lee, Alexander Kurosky, George H. Greeley

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

    Original languageEnglish (US)
    Pages (from-to)123-127
    Number of pages5
    JournalRegulatory Peptides
    Volume83
    Issue number2-3
    DOIs
    StatePublished - Sep 15 1999

    Fingerprint

    Proprotein Convertase 1
    Chromogranin A
    Cells
    Messenger RNA
    Protein Precursors
    Carcinoid Tumor
    High performance liquid chromatography
    Processing
    pancreastatin
    High Pressure Liquid Chromatography
    Cell Line
    Peptides

    Keywords

    • Antisense
    • Endoprotease
    • Peptide
    • Processing

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Neuroscience(all)

    Cite this

    Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin. / Udupi, Vidyavathi; Lee, Heung Man; Kurosky, Alexander; Greeley, George H.

    In: Regulatory Peptides, Vol. 83, No. 2-3, 15.09.1999, p. 123-127.

    Research output: Contribution to journalArticle

    Udupi, Vidyavathi ; Lee, Heung Man ; Kurosky, Alexander ; Greeley, George H. / Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin. In: Regulatory Peptides. 1999 ; Vol. 83, No. 2-3. pp. 123-127.
    @article{4f2da6a411cd4b368655fdbedc600db1,
    title = "Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin",
    abstract = "The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95{\%} reduction) and an 80{\%} reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.",
    keywords = "Antisense, Endoprotease, Peptide, Processing",
    author = "Vidyavathi Udupi and Lee, {Heung Man} and Alexander Kurosky and Greeley, {George H.}",
    year = "1999",
    month = "9",
    day = "15",
    doi = "10.1016/S0167-0115(99)00061-0",
    language = "English (US)",
    volume = "83",
    pages = "123--127",
    journal = "Regulatory Peptides",
    issn = "0167-0115",
    publisher = "Elsevier",
    number = "2-3",

    }

    TY - JOUR

    T1 - Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin

    AU - Udupi, Vidyavathi

    AU - Lee, Heung Man

    AU - Kurosky, Alexander

    AU - Greeley, George H.

    PY - 1999/9/15

    Y1 - 1999/9/15

    N2 - The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

    AB - The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

    KW - Antisense

    KW - Endoprotease

    KW - Peptide

    KW - Processing

    UR - http://www.scopus.com/inward/record.url?scp=0032801426&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0032801426&partnerID=8YFLogxK

    U2 - 10.1016/S0167-0115(99)00061-0

    DO - 10.1016/S0167-0115(99)00061-0

    M3 - Article

    VL - 83

    SP - 123

    EP - 127

    JO - Regulatory Peptides

    JF - Regulatory Peptides

    SN - 0167-0115

    IS - 2-3

    ER -