Proinflammatory cytokines depress cardiac efficiency by a nitric oxide-dependent mechanism

Donna Panas, Fadi H. Khadour, Csaba Szabo, Richard Schulz

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Proinflammatory cytokines (interleukin-1β, tumor necrosis factor-α, and interferon-γ; Cytomix) depress myocardial contractile work partially by stimulating expression of inducible nitric oxide (NO) synthase (iNOS). Because NO and peroxynitrite inhibit myocardial O2 consumption (MV̇O2), we examined whether this mechanism contributes to reduced cardiac work. In control isolated working rat hearts, cardiac work was stable for 60 min, followed by a decline from 60 to 120 min, without change in MV̇O2. Cardiac efficiency (work/MV̇O2) was therefore reduced from 60 to 120 min. Cytomix shortened the onset (within 20-40 min) and enhanced the depression in cardiac work and efficiency and inhibited MV̇O2 after 80 min. Mercaptoethylguanidine (MEG), an iNOS inhibitor and peroxynitrite scavenger, or the glucocorticoid dexamethasone (Dex) abolished the effects of Cytomix. iNOS expression was increased 10-fold by Cytomix and abolished by Dex but not MEG. That cytokine-induced depression in cardiac work precedes the reduction in MV̇O2 suggests, at least in the early response, that NO and/or peroxynitrite may not impair heart function by inhibiting mitochondrial respiration but reduce the heart's ability to utilize ATP for contractile work.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume44
Issue number3
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Nitric Oxide
Cytokines
Peroxynitrous Acid
Dexamethasone
Nitric Oxide Synthase Type II
Interleukin-1
Interferons
Glucocorticoids
Respiration
Tumor Necrosis Factor-alpha
Adenosine Triphosphate
2-mercaptoethylguanidine

Keywords

  • Dexamethasone
  • Inducible nitric oxide synthase
  • Isolated heart
  • Mercaptoethylguanidine
  • Peroxynitrite

ASJC Scopus subject areas

  • Physiology

Cite this

Proinflammatory cytokines depress cardiac efficiency by a nitric oxide-dependent mechanism. / Panas, Donna; Khadour, Fadi H.; Szabo, Csaba; Schulz, Richard.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 44, No. 3, 09.1998.

Research output: Contribution to journalArticle

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