Proinflammatory cytokines in granulomas associated with murine cysticercosis are not the cause of seizures

Shripad Patil, Prema Robinson, Jeffrey K. Actor, Salman Baig, A. Clinton White

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Neurocysticercosis is a parasitic infection of the human central nervous system caused by the cestode Taenia solium. The most common clinical manifestations of neurocysticercosis are seizures. Taenia crassiceps cysticercosis in mice has been used as an experimental model for T. solium cysticercosis. Granulomas surrounding murine cysticerci have striking immunopathological resemblance to human neurocysticercosis; early stage granulomas were able to induce seizures in a rodent model. To assess the role of proinflammatory cytokines in early stage granulomas, we isolated RNA from murine cysticercal granulomas and checked for cytokine expression by reverse transcriptase-polymerase chain reaction (RT-PCR) and/or ribonuclease (RNase) protection assays. Cytokine expression was compared with histological stages. Interleukin (IL)-1α, IL-1β, IL-1 receptor antagonist, and tumor necrosis factor (TNF-α) were the major cytokines detected in all granulomas. Signals for IL-12, IL-18, and IL-6 RNA were not consistently detected and, when detected, were barely demonstrable. Expression of migration inhibitory factor (MIF), IL-6, IL-1α, TNF-α, and IL-18 was not significantly different between early and late-stage granulomas. Expression of IL-1β, IL-1 receptor antagonist, and IL-12 p40 were higher in late, compared with early, stages. Thus, we demonstrated a broad range of cytokines in these granulomas. However, we did not document preferential expression of any proinflammatory cytokines in early stage granulomas. Thus, proinflammatory cytokines are not responsible for the seizures in the rodent model of neurocysticercosis.

Original languageEnglish (US)
Pages (from-to)738-741
Number of pages4
JournalJournal of Parasitology
Volume92
Issue number4
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

Cysticercosis
cysticercosis
granuloma
seizures
Granuloma
interleukin-1
Seizures
neurocysticercosis
cytokines
Cytokines
Neurocysticercosis
tumor
rodent
RNA
mice
Interleukin-1
Taenia solium
nervous system
interleukin-18
polymerase chain reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Proinflammatory cytokines in granulomas associated with murine cysticercosis are not the cause of seizures. / Patil, Shripad; Robinson, Prema; Actor, Jeffrey K.; Baig, Salman; White, A. Clinton.

In: Journal of Parasitology, Vol. 92, No. 4, 08.2006, p. 738-741.

Research output: Contribution to journalArticle

Patil, Shripad ; Robinson, Prema ; Actor, Jeffrey K. ; Baig, Salman ; White, A. Clinton. / Proinflammatory cytokines in granulomas associated with murine cysticercosis are not the cause of seizures. In: Journal of Parasitology. 2006 ; Vol. 92, No. 4. pp. 738-741.
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