Projection structure of photosystem II in vivo determined by Cryo-electron crystallography

Svetla S. Stoylova, Toby D. Flint, Robert C. Ford, Andreas Holzenburg

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Photosystem II (PSII) is a protein-pigment complex situated in the thylakoid membranes of plants and cyanobacteria where it catalyses the conversion of light into chemical energy. This energy is used to extract electrons from water, during which process oxygen is evolved. Owing to its extreme fragility and the large number of polypeptides (> 20) it is composed of, the complex has so far proven recalcitrant to high-resolution structural studies. Cryo-electron crystallography of 2-D crystals (a=15.4 nm, b=23.1 nm, γ=97.2°, pl) comprising in situ PSII revealed the first projection structure of the native complex. The unit cell contains one monomeric complex in which three central domains straddle an elongated intramolecular cavity. In conjunction with earlier data, these central domains were assigned to the reaction center core subunits of PSII consisting of CP43, CP47, the reaction center heterodimer D1/D2 and cytochrome b-559. The data are discussed in view of the evolution of reaction centers from anoxygenic to oxygenic photosynthesis.

Original languageEnglish (US)
Pages (from-to)439-446
Number of pages8
JournalMicron
Volume28
Issue number6
DOIs
StatePublished - Dec 1997
Externally publishedYes

Fingerprint

Crystallography
Photosystem II Protein Complex
crystallography
projection
Electrons
Proteins
Photosynthesis
Polypeptides
Pigments
chemical energy
Thylakoids
electrons
photosynthesis
cytochromes
polypeptides
Cyanobacteria
pigments
Membranes
Crystals
Oxygen

Keywords

  • 2-D crystals
  • Barley
  • Cryo-electron crystallography
  • Photosystem II
  • Structure

ASJC Scopus subject areas

  • Cell Biology
  • Materials Science(all)
  • Instrumentation

Cite this

Projection structure of photosystem II in vivo determined by Cryo-electron crystallography. / Stoylova, Svetla S.; Flint, Toby D.; Ford, Robert C.; Holzenburg, Andreas.

In: Micron, Vol. 28, No. 6, 12.1997, p. 439-446.

Research output: Contribution to journalArticle

Stoylova, Svetla S. ; Flint, Toby D. ; Ford, Robert C. ; Holzenburg, Andreas. / Projection structure of photosystem II in vivo determined by Cryo-electron crystallography. In: Micron. 1997 ; Vol. 28, No. 6. pp. 439-446.
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