Prolactin gene expression in human ovarian follicular cells

John Phelps, Elizabeth M. Bugg, Michael J. Shamblott, Nikos P. Vlahos, Joe Whelan, Howard A. Zacur

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: To investigate prolactin gene expression in human ovarian follicular cells. Design: RNA was isolated from follicular cells obtained at the time of transvaginal oocyte retrieval from patients after controlled ovarian hyperstimulation. The RNA was subjected to reverse transcription and polymerase chain reaction (RT-PCR) using prolactin-specific primers. The RT-PCR products were analyzed by gel electrophoresis, followed by Southern blot analysis using prolactin-specific probes. Setting: Department of gynecology and obstetrics research laboratory. Patient(s): Women undergoing controlled ovarian hyperstimulation followed by transvaginal oocyte retrieval. Intervention(s): Controlled ovarian hyperstimulation followed by transvaginal oocyte retrieval. Main outcome measure(s): Expression of prolactin mRNA by follicular cells. Result(s): The presence of prolactin-specific cDNA amplified by RT-PCR from RNA isolated from human follicular cells was confirmed by Southern blot analysis. Conclusion(s): Human ovarian follicular cells are an extrapituitary site of prolactin gene expression.

Original languageEnglish (US)
Pages (from-to)182-185
Number of pages4
JournalFertility and Sterility
Volume79
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Prolactin
Oocyte Retrieval
Gene Expression
Reverse Transcription
RNA
Southern Blotting
Polymerase Chain Reaction
Hospital Obstetrics and Gynecology Department
Gynecology
Electrophoresis
Complementary DNA
Gels
Outcome Assessment (Health Care)
Messenger RNA
Research

Keywords

  • Follicular cells
  • Ovary
  • Prolactin
  • Transcription

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Phelps, J., Bugg, E. M., Shamblott, M. J., Vlahos, N. P., Whelan, J., & Zacur, H. A. (2003). Prolactin gene expression in human ovarian follicular cells. Fertility and Sterility, 79(1), 182-185. https://doi.org/10.1016/S0015-0282(02)04536-3

Prolactin gene expression in human ovarian follicular cells. / Phelps, John; Bugg, Elizabeth M.; Shamblott, Michael J.; Vlahos, Nikos P.; Whelan, Joe; Zacur, Howard A.

In: Fertility and Sterility, Vol. 79, No. 1, 01.01.2003, p. 182-185.

Research output: Contribution to journalArticle

Phelps, J, Bugg, EM, Shamblott, MJ, Vlahos, NP, Whelan, J & Zacur, HA 2003, 'Prolactin gene expression in human ovarian follicular cells', Fertility and Sterility, vol. 79, no. 1, pp. 182-185. https://doi.org/10.1016/S0015-0282(02)04536-3
Phelps J, Bugg EM, Shamblott MJ, Vlahos NP, Whelan J, Zacur HA. Prolactin gene expression in human ovarian follicular cells. Fertility and Sterility. 2003 Jan 1;79(1):182-185. https://doi.org/10.1016/S0015-0282(02)04536-3
Phelps, John ; Bugg, Elizabeth M. ; Shamblott, Michael J. ; Vlahos, Nikos P. ; Whelan, Joe ; Zacur, Howard A. / Prolactin gene expression in human ovarian follicular cells. In: Fertility and Sterility. 2003 ; Vol. 79, No. 1. pp. 182-185.
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