Abstract
Immunization of genetically susceptible strains of mice with mouse thyroglobulin (MTg) and adjuvant results in sensitization of MTg-specific CD4+ T cells that can induce experimental autoimmune thyroiditis (EAT) after in vitro activation with MTg. These MTg-specific CD4+ T cells also provide help to MTg-specific B cells which produce anti-MTg autoantibodies in vivo. This study was undertaken to develop a system with which to measure MTg-specific helper function in vitro. MTg-activated T cells were shown to provide help to MTg-primed B cells in vitro as assessed by B-cell proliferation and anti-MTg autoantibody production. These responses were MTg-specific and required MTg priming of both the T- and B-cell populations. An MTg-specific CD4+ T-cell line also induced MTg-specific B-cell proliferation and was capable of inducing IgG1, IgG2a and IgG2b synthesis in MTg-primed B cells.
Original language | English (US) |
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Pages (from-to) | 189-193 |
Number of pages | 5 |
Journal | Immunology Letters |
Volume | 45 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1995 |
Externally published | Yes |
Keywords
- Autoantibody production
- B-cell activation
- Mouse thyroglobulin
- T-helper cell
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology