Proliferation and autoantibody production by mouse thyroglobulin (MTg)-specific B cells activated in vitro by MTg and MTg-specific T cells

Yingzi Cong, Helen Braley-Mullen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Immunization of genetically susceptible strains of mice with mouse thyroglobulin (MTg) and adjuvant results in sensitization of MTg-specific CD4+ T cells that can induce experimental autoimmune thyroiditis (EAT) after in vitro activation with MTg. These MTg-specific CD4+ T cells also provide help to MTg-specific B cells which produce anti-MTg autoantibodies in vivo. This study was undertaken to develop a system with which to measure MTg-specific helper function in vitro. MTg-activated T cells were shown to provide help to MTg-primed B cells in vitro as assessed by B-cell proliferation and anti-MTg autoantibody production. These responses were MTg-specific and required MTg priming of both the T- and B-cell populations. An MTg-specific CD4+ T-cell line also induced MTg-specific B-cell proliferation and was capable of inducing IgG1, IgG2a and IgG2b synthesis in MTg-primed B cells.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalImmunology Letters
Volume45
Issue number3
DOIs
StatePublished - Mar 1995
Externally publishedYes

Keywords

  • Autoantibody production
  • B-cell activation
  • Mouse thyroglobulin
  • T-helper cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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