Proliferation and hepatic differentiation of adult-derived progenitor cells

Jian Wang, J. Brian Clark, Gyu Seek Rhee, Jeffrey Fair, Lola M. Reid, David A. Gerber

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Hepatic progenitor cells, capable of maturing into hepatocytes and biliary cells, are hypothesized to be involved in all forms of liver regeneration and may prove clinically useful at reconstituting damaged livers. A murine hepatic progenitor cell population from young adult liver tissue has been isolated and characterized to establish a model for the development of liver cell therapies and for analysis of immune responses after transplantation. Hepatic progenitor cells were isolated from 3- to 6-week-old C57BL/6 mice using modifications of a two-stage liver perfusion technique followed by low speed centrifugation. Cellular analysis by phase contrast, fluorescent and confocal microscopy demonstrated that the hepatic progenitors (1) formed ex vivo colonies with a morphological appearance similar to committed hepatocytic progenitors isolated from embryonic mice and rats; (2) they are smaller than mature hepatocytes; (3) in culture they demonstrated peak expression of an oval cell marker at day 14, whereas albumin expression continued to increase beyond day 21 of culture, and (4) a subset of the progenitors phenotypically differentiated into mature hepatocytes or biliary cells. The unique antigenic profile of these hepatic progenitor cells and their ability to differentiate suggests that purification of the cells should allow for their potential use in transplantation.

Original languageEnglish (US)
Pages (from-to)193-203
Number of pages11
JournalCells Tissues Organs
Volume173
Issue number4
DOIs
StatePublished - 2003
Externally publishedYes

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Hepatocytes
Stem Cells
Liver
Transplantation
Phase-Contrast Microscopy
Liver Regeneration
Cell- and Tissue-Based Therapy
Centrifugation
Inbred C57BL Mouse
Confocal Microscopy
Young Adult
Albumins
Perfusion
Population

Keywords

  • A6
  • Hepatocyte
  • Liver
  • Mouse
  • Stem cell

ASJC Scopus subject areas

  • Anatomy

Cite this

Wang, J., Clark, J. B., Rhee, G. S., Fair, J., Reid, L. M., & Gerber, D. A. (2003). Proliferation and hepatic differentiation of adult-derived progenitor cells. Cells Tissues Organs, 173(4), 193-203. https://doi.org/10.1159/000070375

Proliferation and hepatic differentiation of adult-derived progenitor cells. / Wang, Jian; Clark, J. Brian; Rhee, Gyu Seek; Fair, Jeffrey; Reid, Lola M.; Gerber, David A.

In: Cells Tissues Organs, Vol. 173, No. 4, 2003, p. 193-203.

Research output: Contribution to journalArticle

Wang, J, Clark, JB, Rhee, GS, Fair, J, Reid, LM & Gerber, DA 2003, 'Proliferation and hepatic differentiation of adult-derived progenitor cells', Cells Tissues Organs, vol. 173, no. 4, pp. 193-203. https://doi.org/10.1159/000070375
Wang, Jian ; Clark, J. Brian ; Rhee, Gyu Seek ; Fair, Jeffrey ; Reid, Lola M. ; Gerber, David A. / Proliferation and hepatic differentiation of adult-derived progenitor cells. In: Cells Tissues Organs. 2003 ; Vol. 173, No. 4. pp. 193-203.
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