Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model

J. D. Blanco, T. S. Wen, K. Bishop, D. A. Eschenbach, J. A. Hill, G. W. Wilbanks

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: Our purpose was to compare the rate of adverse pregnancy outcome in pregnant mice with lower genital tract chlamydial infection who had a prior short chlamydial infection versus a prior long-term infection. STUDY DESIGN: A total of 127 female mice were divided into short-term and long-term infection groups. We infected the lower genital tracts with Chlamydia trachomatis. After 7 days in the short-term infection group and 30 days in the long-term infection group, we treated the mice with tetracycline- impregnated chow. After documentation of cure, the mice were mated and transvaginally reinfected with Chlamydia trachomatis. Forty-one of the 127 (32%) mice became pregnant. We noted the number of mice with fetal death and the number of pups present. We cultured the lower uterine segment and the pups for Chlamydia. RESULTS: Seven of 21 (33%) mice in the short-term infection group had fetal deaths compared with 1 of 20 (5%) in the long-term infection group (p < 0.05). In the short-term infection group 21 of 21 (100%) mice had positive transvaginal chlamydial cultures after reinoculation compared with only 7 of 20 (35%) in the long-term infection group (p < 0.000004). Seventeen of 21 (81%) mice in the short-term infection group had positive chlamydial cultures from the lower uterine segment versus 1 of 20 (5%) in the long-term infection group (p < 0.000001). Sixty-five percent of pups in the short-term infection group and none (0%) of the pups in the long- term infection group were positive for Chlamydia (p < 0.00001). CONCLUSIONS: We conclude that in this murine model a prior 30-day genital tract infection with Chlamydia protects pregnant mice from subsequent reinfection and adverse pregnancy outcomes.

Original languageEnglish (US)
Pages (from-to)745-750
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume176
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Chlamydia Infections
Pregnancy Outcome
Infection
Chlamydia
Reproductive Tract Infections
Fetal Death
Chlamydia trachomatis
Tetracycline
Documentation

Keywords

  • Chlamydia trachomatis
  • murine model
  • pregnancy outcome

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Blanco, J. D., Wen, T. S., Bishop, K., Eschenbach, D. A., Hill, J. A., & Wilbanks, G. W. (1997). Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model. American Journal of Obstetrics and Gynecology, 176(4), 745-750.

Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model. / Blanco, J. D.; Wen, T. S.; Bishop, K.; Eschenbach, D. A.; Hill, J. A.; Wilbanks, G. W.

In: American Journal of Obstetrics and Gynecology, Vol. 176, No. 4, 1997, p. 745-750.

Research output: Contribution to journalArticle

Blanco, JD, Wen, TS, Bishop, K, Eschenbach, DA, Hill, JA & Wilbanks, GW 1997, 'Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model', American Journal of Obstetrics and Gynecology, vol. 176, no. 4, pp. 745-750.
Blanco, J. D. ; Wen, T. S. ; Bishop, K. ; Eschenbach, D. A. ; Hill, J. A. ; Wilbanks, G. W. / Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model. In: American Journal of Obstetrics and Gynecology. 1997 ; Vol. 176, No. 4. pp. 745-750.
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abstract = "OBJECTIVE: Our purpose was to compare the rate of adverse pregnancy outcome in pregnant mice with lower genital tract chlamydial infection who had a prior short chlamydial infection versus a prior long-term infection. STUDY DESIGN: A total of 127 female mice were divided into short-term and long-term infection groups. We infected the lower genital tracts with Chlamydia trachomatis. After 7 days in the short-term infection group and 30 days in the long-term infection group, we treated the mice with tetracycline- impregnated chow. After documentation of cure, the mice were mated and transvaginally reinfected with Chlamydia trachomatis. Forty-one of the 127 (32{\%}) mice became pregnant. We noted the number of mice with fetal death and the number of pups present. We cultured the lower uterine segment and the pups for Chlamydia. RESULTS: Seven of 21 (33{\%}) mice in the short-term infection group had fetal deaths compared with 1 of 20 (5{\%}) in the long-term infection group (p < 0.05). In the short-term infection group 21 of 21 (100{\%}) mice had positive transvaginal chlamydial cultures after reinoculation compared with only 7 of 20 (35{\%}) in the long-term infection group (p < 0.000004). Seventeen of 21 (81{\%}) mice in the short-term infection group had positive chlamydial cultures from the lower uterine segment versus 1 of 20 (5{\%}) in the long-term infection group (p < 0.000001). Sixty-five percent of pups in the short-term infection group and none (0{\%}) of the pups in the long- term infection group were positive for Chlamydia (p < 0.00001). CONCLUSIONS: We conclude that in this murine model a prior 30-day genital tract infection with Chlamydia protects pregnant mice from subsequent reinfection and adverse pregnancy outcomes.",
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T1 - Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model

AU - Blanco, J. D.

AU - Wen, T. S.

AU - Bishop, K.

AU - Eschenbach, D. A.

AU - Hill, J. A.

AU - Wilbanks, G. W.

PY - 1997

Y1 - 1997

N2 - OBJECTIVE: Our purpose was to compare the rate of adverse pregnancy outcome in pregnant mice with lower genital tract chlamydial infection who had a prior short chlamydial infection versus a prior long-term infection. STUDY DESIGN: A total of 127 female mice were divided into short-term and long-term infection groups. We infected the lower genital tracts with Chlamydia trachomatis. After 7 days in the short-term infection group and 30 days in the long-term infection group, we treated the mice with tetracycline- impregnated chow. After documentation of cure, the mice were mated and transvaginally reinfected with Chlamydia trachomatis. Forty-one of the 127 (32%) mice became pregnant. We noted the number of mice with fetal death and the number of pups present. We cultured the lower uterine segment and the pups for Chlamydia. RESULTS: Seven of 21 (33%) mice in the short-term infection group had fetal deaths compared with 1 of 20 (5%) in the long-term infection group (p < 0.05). In the short-term infection group 21 of 21 (100%) mice had positive transvaginal chlamydial cultures after reinoculation compared with only 7 of 20 (35%) in the long-term infection group (p < 0.000004). Seventeen of 21 (81%) mice in the short-term infection group had positive chlamydial cultures from the lower uterine segment versus 1 of 20 (5%) in the long-term infection group (p < 0.000001). Sixty-five percent of pups in the short-term infection group and none (0%) of the pups in the long- term infection group were positive for Chlamydia (p < 0.00001). CONCLUSIONS: We conclude that in this murine model a prior 30-day genital tract infection with Chlamydia protects pregnant mice from subsequent reinfection and adverse pregnancy outcomes.

AB - OBJECTIVE: Our purpose was to compare the rate of adverse pregnancy outcome in pregnant mice with lower genital tract chlamydial infection who had a prior short chlamydial infection versus a prior long-term infection. STUDY DESIGN: A total of 127 female mice were divided into short-term and long-term infection groups. We infected the lower genital tracts with Chlamydia trachomatis. After 7 days in the short-term infection group and 30 days in the long-term infection group, we treated the mice with tetracycline- impregnated chow. After documentation of cure, the mice were mated and transvaginally reinfected with Chlamydia trachomatis. Forty-one of the 127 (32%) mice became pregnant. We noted the number of mice with fetal death and the number of pups present. We cultured the lower uterine segment and the pups for Chlamydia. RESULTS: Seven of 21 (33%) mice in the short-term infection group had fetal deaths compared with 1 of 20 (5%) in the long-term infection group (p < 0.05). In the short-term infection group 21 of 21 (100%) mice had positive transvaginal chlamydial cultures after reinoculation compared with only 7 of 20 (35%) in the long-term infection group (p < 0.000004). Seventeen of 21 (81%) mice in the short-term infection group had positive chlamydial cultures from the lower uterine segment versus 1 of 20 (5%) in the long-term infection group (p < 0.000001). Sixty-five percent of pups in the short-term infection group and none (0%) of the pups in the long- term infection group were positive for Chlamydia (p < 0.00001). CONCLUSIONS: We conclude that in this murine model a prior 30-day genital tract infection with Chlamydia protects pregnant mice from subsequent reinfection and adverse pregnancy outcomes.

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