Propagation and preliminary characterization of a chicken candidate calicivirus

W. D. Cubitt, Alan Barrett

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present paper reports the propagation and partial characterization of calici-like virus particles previously observed in gut homogenates obtained from stunted broiler chicks. Radiolabelling experiments with [3H]uridine showed that the virus possesses an RNA genome and that it can replicate in primary chick embryo fibroblasts in the presence of actinomycin D. In vivo studies showed that the virus can be serially passaged in specific pathogen-free chicks and virus particles can be recovered from the gut and excreta of infected birds. The morphological appearance and biophysical properties of the virus were similar to those of feline calicivirus, which supports the view that it should be tentatively classified as a member of the Caliciviridae.

Original languageEnglish (US)
Pages (from-to)1431-1438
Number of pages8
JournalJournal of General Virology
Volume66
Issue number7
StatePublished - 1985
Externally publishedYes

Fingerprint

Virion
Chickens
Caliciviridae
Feline Calicivirus
Viruses
Specific Pathogen-Free Organisms
Uridine
RNA Viruses
Dactinomycin
Chick Embryo
Birds
Fibroblasts
Genome

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Propagation and preliminary characterization of a chicken candidate calicivirus. / Cubitt, W. D.; Barrett, Alan.

In: Journal of General Virology, Vol. 66, No. 7, 1985, p. 1431-1438.

Research output: Contribution to journalArticle

@article{a7f11886d265433886a4c06ee29af3be,
title = "Propagation and preliminary characterization of a chicken candidate calicivirus",
abstract = "The present paper reports the propagation and partial characterization of calici-like virus particles previously observed in gut homogenates obtained from stunted broiler chicks. Radiolabelling experiments with [3H]uridine showed that the virus possesses an RNA genome and that it can replicate in primary chick embryo fibroblasts in the presence of actinomycin D. In vivo studies showed that the virus can be serially passaged in specific pathogen-free chicks and virus particles can be recovered from the gut and excreta of infected birds. The morphological appearance and biophysical properties of the virus were similar to those of feline calicivirus, which supports the view that it should be tentatively classified as a member of the Caliciviridae.",
author = "Cubitt, {W. D.} and Alan Barrett",
year = "1985",
language = "English (US)",
volume = "66",
pages = "1431--1438",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "7",

}

TY - JOUR

T1 - Propagation and preliminary characterization of a chicken candidate calicivirus

AU - Cubitt, W. D.

AU - Barrett, Alan

PY - 1985

Y1 - 1985

N2 - The present paper reports the propagation and partial characterization of calici-like virus particles previously observed in gut homogenates obtained from stunted broiler chicks. Radiolabelling experiments with [3H]uridine showed that the virus possesses an RNA genome and that it can replicate in primary chick embryo fibroblasts in the presence of actinomycin D. In vivo studies showed that the virus can be serially passaged in specific pathogen-free chicks and virus particles can be recovered from the gut and excreta of infected birds. The morphological appearance and biophysical properties of the virus were similar to those of feline calicivirus, which supports the view that it should be tentatively classified as a member of the Caliciviridae.

AB - The present paper reports the propagation and partial characterization of calici-like virus particles previously observed in gut homogenates obtained from stunted broiler chicks. Radiolabelling experiments with [3H]uridine showed that the virus possesses an RNA genome and that it can replicate in primary chick embryo fibroblasts in the presence of actinomycin D. In vivo studies showed that the virus can be serially passaged in specific pathogen-free chicks and virus particles can be recovered from the gut and excreta of infected birds. The morphological appearance and biophysical properties of the virus were similar to those of feline calicivirus, which supports the view that it should be tentatively classified as a member of the Caliciviridae.

UR - http://www.scopus.com/inward/record.url?scp=0021808058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021808058&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 1431

EP - 1438

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 7

ER -