Propagation and titration of west Nile virus on Vero cells

Alexander J. McAuley, David Beasley

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The propagation and titration of viruses are key virological techniques. Unlike other flaviviruses, such as the dengue viruses, West Nile virus (WNV) grows and plaques very efficiently on Vero cells, usually inducing strong cytopathic effect (CPE) and forming clear plaques. Here, we outline the steps for propagating WNV from culture supernatant stocks and homogenized organ/mosquito samples, as well as for determining virus titers in samples by serial-dilution plaque assay using neutral red or crystal violet stains.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-27
Number of pages9
Volume1435
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1435
ISSN (Print)10643745

Fingerprint

West Nile virus
Vero Cells
Gentian Violet
Neutral Red
Flavivirus
Dengue Virus
Culicidae
Viral Load
Coloring Agents
Viruses

Keywords

  • Plaque assay
  • Vero cells
  • Virus propagation
  • Virus titration
  • West Nile virus

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

McAuley, A. J., & Beasley, D. (2016). Propagation and titration of west Nile virus on Vero cells. In Methods in Molecular Biology (Vol. 1435, pp. 19-27). (Methods in Molecular Biology; Vol. 1435). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3670-0_3

Propagation and titration of west Nile virus on Vero cells. / McAuley, Alexander J.; Beasley, David.

Methods in Molecular Biology. Vol. 1435 Humana Press Inc., 2016. p. 19-27 (Methods in Molecular Biology; Vol. 1435).

Research output: Chapter in Book/Report/Conference proceedingChapter

McAuley, AJ & Beasley, D 2016, Propagation and titration of west Nile virus on Vero cells. in Methods in Molecular Biology. vol. 1435, Methods in Molecular Biology, vol. 1435, Humana Press Inc., pp. 19-27. https://doi.org/10.1007/978-1-4939-3670-0_3
McAuley AJ, Beasley D. Propagation and titration of west Nile virus on Vero cells. In Methods in Molecular Biology. Vol. 1435. Humana Press Inc. 2016. p. 19-27. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3670-0_3
McAuley, Alexander J. ; Beasley, David. / Propagation and titration of west Nile virus on Vero cells. Methods in Molecular Biology. Vol. 1435 Humana Press Inc., 2016. pp. 19-27 (Methods in Molecular Biology).
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