Propagation and titration of west Nile virus on Vero cells

Alexander J. McAuley, David W C Beasley

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The propagation and titration of viruses are key virological techniques. Unlike other flaviviruses, such as the dengue viruses, West Nile virus (WNV) grows and plaques very efficiently on Vero cells, usually inducing strong cytopathic effect (CPE) and forming clear plaques. Here, we outline the steps for propagating WNV from culture supernatant stocks and homogenized organ/mosquito samples, as well as for determining virus titers in samples by serial-dilution plaque assay using neutral red or crystal violet stains.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-27
Number of pages9
Volume1435
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1435
ISSN (Print)10643745

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Keywords

  • Plaque assay
  • Vero cells
  • Virus propagation
  • Virus titration
  • West Nile virus

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

McAuley, A. J., & Beasley, D. W. C. (2016). Propagation and titration of west Nile virus on Vero cells. In Methods in Molecular Biology (Vol. 1435, pp. 19-27). (Methods in Molecular Biology; Vol. 1435). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3670-0_3