Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica

Pierre Edouard Fournier, Yong Zhu, Xuejie Yu, Didier Raoult

Research output: Chapter in Book/Report/Conference proceedingConference contribution

28 Citations (Scopus)

Abstract

The Rickettsia sibirica species is composed of isolates that are genotypically close but can be classified within two distinct serotypes, that is, R. sibirica sensu stricto and R. sibirica mongolitimonae (incorrectly named R. mongolotimonae). We investigated the possibility of classifying rickettsiae closely related to R. sibirica as R. sibirica subspecies, as proposed by the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. For this, we first estimated the genotypic variability by using multilocus sequence typing (MLST), including the sequencing of five genes, and multispacer typing (MST) using three intergenic spacers, of five isolates and three tick amplicons of R. sibirica sensu stricto and six isolates of A. sibirica mongolotimonae. Then, we selected a representative of each MLST genotype and used mouse serotyping to estimate their degree of taxonomic relatedness. Among the 14 isolates or tick amplicons studied, 2 MLST genotypes were identified: (i) the R. sibirica sensu stricto type; and (ii) the R. sibirica mongolitimonae type. Representatives of the two MLST types were classified within three MST types and into two serotypes. Therefore, as isolates within the R. sibirica species are genotypically homogeneous but show MST genotypic, serotypic, and epidemio-clinical dissimilarities, we propose to modify the nomenclature of the R. sibirica species through the creation of subspecies. We propose the names R. sibirica subsp. sibirica subsp. nov. (type strain = 2-4-6, ATCC VR-541 T), and R. sibirica subsp. mongolitimonae subsp. nov. (type strain = HA-91, ATCC VR-1526T). The description of A. sibirica is emended to accommodate the two subspecies.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages597-606
Number of pages10
Volume1078
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1078
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

aprepitant
Multilocus Sequence Typing
Rickettsia
Ticks
Terminology
Genes
Genotype
Serotyping
Names

Keywords

  • Rickettsia siberica
  • Subspecies
  • Taxonomy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fournier, P. E., Zhu, Y., Yu, X., & Raoult, D. (2006). Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica. In Annals of the New York Academy of Sciences (Vol. 1078, pp. 597-606). (Annals of the New York Academy of Sciences; Vol. 1078). https://doi.org/10.1196/annals.1374.120

Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica. / Fournier, Pierre Edouard; Zhu, Yong; Yu, Xuejie; Raoult, Didier.

Annals of the New York Academy of Sciences. Vol. 1078 2006. p. 597-606 (Annals of the New York Academy of Sciences; Vol. 1078).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fournier, PE, Zhu, Y, Yu, X & Raoult, D 2006, Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica. in Annals of the New York Academy of Sciences. vol. 1078, Annals of the New York Academy of Sciences, vol. 1078, pp. 597-606. https://doi.org/10.1196/annals.1374.120
Fournier PE, Zhu Y, Yu X, Raoult D. Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica. In Annals of the New York Academy of Sciences. Vol. 1078. 2006. p. 597-606. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1374.120
Fournier, Pierre Edouard ; Zhu, Yong ; Yu, Xuejie ; Raoult, Didier. / Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica. Annals of the New York Academy of Sciences. Vol. 1078 2006. pp. 597-606 (Annals of the New York Academy of Sciences).
@inproceedings{80fd2d873b4847ccb3e0bcfcd006899b,
title = "Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica",
abstract = "The Rickettsia sibirica species is composed of isolates that are genotypically close but can be classified within two distinct serotypes, that is, R. sibirica sensu stricto and R. sibirica mongolitimonae (incorrectly named R. mongolotimonae). We investigated the possibility of classifying rickettsiae closely related to R. sibirica as R. sibirica subspecies, as proposed by the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. For this, we first estimated the genotypic variability by using multilocus sequence typing (MLST), including the sequencing of five genes, and multispacer typing (MST) using three intergenic spacers, of five isolates and three tick amplicons of R. sibirica sensu stricto and six isolates of A. sibirica mongolotimonae. Then, we selected a representative of each MLST genotype and used mouse serotyping to estimate their degree of taxonomic relatedness. Among the 14 isolates or tick amplicons studied, 2 MLST genotypes were identified: (i) the R. sibirica sensu stricto type; and (ii) the R. sibirica mongolitimonae type. Representatives of the two MLST types were classified within three MST types and into two serotypes. Therefore, as isolates within the R. sibirica species are genotypically homogeneous but show MST genotypic, serotypic, and epidemio-clinical dissimilarities, we propose to modify the nomenclature of the R. sibirica species through the creation of subspecies. We propose the names R. sibirica subsp. sibirica subsp. nov. (type strain = 2-4-6, ATCC VR-541 T), and R. sibirica subsp. mongolitimonae subsp. nov. (type strain = HA-91, ATCC VR-1526T). The description of A. sibirica is emended to accommodate the two subspecies.",
keywords = "Rickettsia siberica, Subspecies, Taxonomy",
author = "Fournier, {Pierre Edouard} and Yong Zhu and Xuejie Yu and Didier Raoult",
year = "2006",
month = "10",
doi = "10.1196/annals.1374.120",
language = "English (US)",
isbn = "1573316393",
volume = "1078",
series = "Annals of the New York Academy of Sciences",
pages = "597--606",
booktitle = "Annals of the New York Academy of Sciences",

}

TY - GEN

T1 - Proposal to create subspecies of Rickettsia sibirica and an emended description of Rickettsia sibirica

AU - Fournier, Pierre Edouard

AU - Zhu, Yong

AU - Yu, Xuejie

AU - Raoult, Didier

PY - 2006/10

Y1 - 2006/10

N2 - The Rickettsia sibirica species is composed of isolates that are genotypically close but can be classified within two distinct serotypes, that is, R. sibirica sensu stricto and R. sibirica mongolitimonae (incorrectly named R. mongolotimonae). We investigated the possibility of classifying rickettsiae closely related to R. sibirica as R. sibirica subspecies, as proposed by the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. For this, we first estimated the genotypic variability by using multilocus sequence typing (MLST), including the sequencing of five genes, and multispacer typing (MST) using three intergenic spacers, of five isolates and three tick amplicons of R. sibirica sensu stricto and six isolates of A. sibirica mongolotimonae. Then, we selected a representative of each MLST genotype and used mouse serotyping to estimate their degree of taxonomic relatedness. Among the 14 isolates or tick amplicons studied, 2 MLST genotypes were identified: (i) the R. sibirica sensu stricto type; and (ii) the R. sibirica mongolitimonae type. Representatives of the two MLST types were classified within three MST types and into two serotypes. Therefore, as isolates within the R. sibirica species are genotypically homogeneous but show MST genotypic, serotypic, and epidemio-clinical dissimilarities, we propose to modify the nomenclature of the R. sibirica species through the creation of subspecies. We propose the names R. sibirica subsp. sibirica subsp. nov. (type strain = 2-4-6, ATCC VR-541 T), and R. sibirica subsp. mongolitimonae subsp. nov. (type strain = HA-91, ATCC VR-1526T). The description of A. sibirica is emended to accommodate the two subspecies.

AB - The Rickettsia sibirica species is composed of isolates that are genotypically close but can be classified within two distinct serotypes, that is, R. sibirica sensu stricto and R. sibirica mongolitimonae (incorrectly named R. mongolotimonae). We investigated the possibility of classifying rickettsiae closely related to R. sibirica as R. sibirica subspecies, as proposed by the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. For this, we first estimated the genotypic variability by using multilocus sequence typing (MLST), including the sequencing of five genes, and multispacer typing (MST) using three intergenic spacers, of five isolates and three tick amplicons of R. sibirica sensu stricto and six isolates of A. sibirica mongolotimonae. Then, we selected a representative of each MLST genotype and used mouse serotyping to estimate their degree of taxonomic relatedness. Among the 14 isolates or tick amplicons studied, 2 MLST genotypes were identified: (i) the R. sibirica sensu stricto type; and (ii) the R. sibirica mongolitimonae type. Representatives of the two MLST types were classified within three MST types and into two serotypes. Therefore, as isolates within the R. sibirica species are genotypically homogeneous but show MST genotypic, serotypic, and epidemio-clinical dissimilarities, we propose to modify the nomenclature of the R. sibirica species through the creation of subspecies. We propose the names R. sibirica subsp. sibirica subsp. nov. (type strain = 2-4-6, ATCC VR-541 T), and R. sibirica subsp. mongolitimonae subsp. nov. (type strain = HA-91, ATCC VR-1526T). The description of A. sibirica is emended to accommodate the two subspecies.

KW - Rickettsia siberica

KW - Subspecies

KW - Taxonomy

UR - http://www.scopus.com/inward/record.url?scp=33845707803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845707803&partnerID=8YFLogxK

U2 - 10.1196/annals.1374.120

DO - 10.1196/annals.1374.120

M3 - Conference contribution

C2 - 17114787

AN - SCOPUS:33845707803

SN - 1573316393

SN - 9781573316392

VL - 1078

T3 - Annals of the New York Academy of Sciences

SP - 597

EP - 606

BT - Annals of the New York Academy of Sciences

ER -