TY - GEN
T1 - Propulsive forces in keratocyte migration
AU - Galbraith, C. G.
AU - Sheetz, M. P.
PY - 1999
Y1 - 1999
N2 - To move forward, migrating cells pull rearward against extracellular matrix and exert traction forces. To elucidate the mechanism of traction force generation in keratocytes, we have measured the forces generated against ECM contacts on both the ventral and the dorsal surfaces of the lamella. We used a micromachined substrate to measure forces on the ventral surface and an optical gradient trap to measure forces on the dorsal surface. Both methods determined comparable forces per unit area that were rearward in the front of the lamella, increasing toward the perinuclear region, and eventually changing direction. The rearward traction forces generated throughout the lamella suggest that myosin condensation at the cell body boundary pulls the lamella actin network rearward and pushes the cell body forward.
AB - To move forward, migrating cells pull rearward against extracellular matrix and exert traction forces. To elucidate the mechanism of traction force generation in keratocytes, we have measured the forces generated against ECM contacts on both the ventral and the dorsal surfaces of the lamella. We used a micromachined substrate to measure forces on the ventral surface and an optical gradient trap to measure forces on the dorsal surface. Both methods determined comparable forces per unit area that were rearward in the front of the lamella, increasing toward the perinuclear region, and eventually changing direction. The rearward traction forces generated throughout the lamella suggest that myosin condensation at the cell body boundary pulls the lamella actin network rearward and pushes the cell body forward.
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M3 - Conference contribution
AN - SCOPUS:0033312027
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 100
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -