Prosimian hemoglobins. III. The primary structures of the duplicated α-globin chains of Lemur variegatus

Dorian H. Coppenhaver, John Buettner-Janusch, Margaret M. Ehrhardt, Lawrence K. Duffy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The amino acid sequences of the α chains of hemoglobins purified from Lemur variegatus erythrocytes have been determined. The sequences were determined primarily from peptides generated from treatment of the isolated α chains with cyanogen bromide or warm formic acid. The ordering of the peptides from both α globins was based on the homology between lemur hemoglobins and those of other primates. The genetic difference at position 15 (Asn vs. Lys) explains the phenotypic characteristic of two hemoglobin species during alkaline electrophoresis. The function of certain residues is discussed in the context of other known sequences. The dispersion of the amino acid changes noted in lemur species falls mostly within the first 75 residues of the α chain (exons 1 and 2). The extent of divergence of the L. variegatus α-globin chains from the Lemur fulvus α globin is similar to that seen for the β-globin chains of these species. This degree of separation (11-16 residues) is consistent with an extended period of independent evolution by these congeneric species after their divergence.

Original languageEnglish (US)
Pages (from-to)372-378
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume873
Issue number3
DOIs
StatePublished - Oct 17 1986

Fingerprint

Lemur
Strepsirhini
Globins
Hemoglobins
formic acid
Amino Acids
Cyanogen Bromide
Peptides
Electrophoresis
Primates
Amino Acid Sequence
Exons
Erythrocytes

Keywords

  • (Lemur variegatus)
  • Amino acid sequence
  • Covarion
  • Gene duplication
  • Hemoglobin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology
  • Medicine(all)

Cite this

Prosimian hemoglobins. III. The primary structures of the duplicated α-globin chains of Lemur variegatus. / Coppenhaver, Dorian H.; Buettner-Janusch, John; Ehrhardt, Margaret M.; Duffy, Lawrence K.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 873, No. 3, 17.10.1986, p. 372-378.

Research output: Contribution to journalArticle

Coppenhaver, Dorian H. ; Buettner-Janusch, John ; Ehrhardt, Margaret M. ; Duffy, Lawrence K. / Prosimian hemoglobins. III. The primary structures of the duplicated α-globin chains of Lemur variegatus. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1986 ; Vol. 873, No. 3. pp. 372-378.
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