Prostaglandin F rises in response to hydroxyl radical generated in vivo

Danxia Liu, Liping Li

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Free radicals and some free fatty acids, such as arachidonic acid metabolites, have been hypothesized to be contributors to secondary damage to the spinal cord upon injury. These two types of species may form a feedback loop in which generation of one type leads to formation of the other. In this study, to determine whether hydroxyl radical causes generation of arachidonic acid metabolites in vivo, we generated hydroxyl radical, a most reactive oxygen radical, in the rat spinal cord and measured resulting changes in levels of prostaglandin F, an arachidonic acid metabolite that rises following traumatic injury. The hydroxyl radical was generated in the rat spinal cord by administering H2O2 through one microdialysis fiber and FeC12/EDTA through a parallel fiber. The prostaglandin F in the collected microdialysates was measured by HPLC as its 3-bromomethyl-6,7-dimethoxy-l-methyl-2-(1H)-quinoxalinone derivative. Prostaglandin F dramatically increased in response to hydroxyl radical generation, but declined substantially after 3 h of exposure. Prostaglandin F was undetectable when either H2O2 or FeC12/EDTA was administered alone in control experiments, demonstrating that its formation was caused by generated hydroxyl radical.

Original languageEnglish (US)
Pages (from-to)571-576
Number of pages6
JournalFree Radical Biology and Medicine
Volume18
Issue number3
DOIs
StatePublished - 1995

Fingerprint

Dinoprost
Hydroxyl Radical
Metabolites
Arachidonic Acid
Edetic Acid
Rats
Spinal Cord
Fibers
Microdialysis
Spinal Cord Injuries
Nonesterified Fatty Acids
Free Radicals
Reactive Oxygen Species
High Pressure Liquid Chromatography
Derivatives
Feedback
Wounds and Injuries
Experiments

Keywords

  • Arachidonic acid metabolites
  • Fenton's reagents
  • Free radicals
  • HPLC analysis
  • Hydroxyl radical generation
  • Microdialysis
  • Prostaglandin F Secondary spinal cord injury

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)
  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Prostaglandin F rises in response to hydroxyl radical generated in vivo. / Liu, Danxia; Li, Liping.

In: Free Radical Biology and Medicine, Vol. 18, No. 3, 1995, p. 571-576.

Research output: Contribution to journalArticle

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