Prostaglandin-induced activation of uterine contractility in pregnant rats does not involve potassium channels

Carolyn A L Bailie, Yuri P. Vedernikov, George Saade, Robert E. Garfield

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVE: The uterus is a target for prostaglandins, especially at the end of gestation. Whether potassium channels are involved in the effect of prostaglandins is not clear. The aim of this study was to find out. STUDY DESIGN: Concentration-response relationships to prostaglandins (prostaglandin F , prostaglandin E 2, and prostaglandin I 2 [carbacyclin]; 10 -10 mol/L-10 -4 mol/L) were studied in isolated uterine rings from mid pregnancy (day 14) and late pregnancy (day 21) rats (Krebs solution, 5% CO 2 in air, 37°C; pH, 7.4). Rings were incubated for 30 minutes with either solvent or adenosine triphosphate - sensitive potassium channel inhibitor or opener glibenclamide and levcromakalim or with calcium-sensitive potassium channel inhibitor or opener NS1619 and iberiotoxin, respectively. The changes in integral activity were compared after each concentration of the agent and were expressed as a percent of the basal integral activity. RESULTS: The increases in spontaneous contractile activity induced by prostaglandin E 2 and carbacyclin, but not prostaglandin F , were statistically significantly higher in tissues from late pregnancy versus mid pregnancy rats and were not affected by any of the K-channel openers or inhibitors. CONCLUSION: Adenosine triphosphate - sensitive and calcium-sensitive potassium channels are not involved in the effect of prostaglandin F , prostaglandin E 2, and prostaglandin I 2 on pregnant rat uterus.

Original languageEnglish (US)
Pages (from-to)453-457
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume186
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Potassium Channels
Prostaglandins
Prostaglandins F
Pregnancy
Prostaglandins E
Epoprostenol
Uterus
Adenosine Triphosphate
Cromakalim
Calcium
Glyburide
Carbon Monoxide
Air
carboprostacyclin

Keywords

  • Potassium channels
  • Prostaglandins
  • Uterine smooth muscle

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Prostaglandin-induced activation of uterine contractility in pregnant rats does not involve potassium channels. / Bailie, Carolyn A L; Vedernikov, Yuri P.; Saade, George; Garfield, Robert E.

In: American Journal of Obstetrics and Gynecology, Vol. 186, No. 3, 2002, p. 453-457.

Research output: Contribution to journalArticle

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AB - OBJECTIVE: The uterus is a target for prostaglandins, especially at the end of gestation. Whether potassium channels are involved in the effect of prostaglandins is not clear. The aim of this study was to find out. STUDY DESIGN: Concentration-response relationships to prostaglandins (prostaglandin F 2α, prostaglandin E 2, and prostaglandin I 2 [carbacyclin]; 10 -10 mol/L-10 -4 mol/L) were studied in isolated uterine rings from mid pregnancy (day 14) and late pregnancy (day 21) rats (Krebs solution, 5% CO 2 in air, 37°C; pH, 7.4). Rings were incubated for 30 minutes with either solvent or adenosine triphosphate - sensitive potassium channel inhibitor or opener glibenclamide and levcromakalim or with calcium-sensitive potassium channel inhibitor or opener NS1619 and iberiotoxin, respectively. The changes in integral activity were compared after each concentration of the agent and were expressed as a percent of the basal integral activity. RESULTS: The increases in spontaneous contractile activity induced by prostaglandin E 2 and carbacyclin, but not prostaglandin F 2α, were statistically significantly higher in tissues from late pregnancy versus mid pregnancy rats and were not affected by any of the K-channel openers or inhibitors. CONCLUSION: Adenosine triphosphate - sensitive and calcium-sensitive potassium channels are not involved in the effect of prostaglandin F 2α, prostaglandin E 2, and prostaglandin I 2 on pregnant rat uterus.

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