Prostaglandin synthesis by enterocyte microsomes of rabbit small intestine

Marwan A. Balaa, Don W. Powell

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We studied the prostaglandin (PG) synthetic capacity of microsomes of a relatively pure population of rabbit enterocytes and determined ideal conditions for product synthesis. The epithelial cells were freed from the basement membrane by a combination of calcium chelation and mechanical vibration, and 100,000 x g microsomes were prepared. These microsomes were found to synthesize PG from exogenously added arachidonic acid. The ideal conditions for the reaction were a microsomal protein concentration of 1.0 mg/ml, an arachidonic acid concentration of 33 μM, a reaction mixture pH of 8.0 - 9.5 and with epinephrine 1.5 mM added as a cofactor. The product yields increased linearly with time up to 30 min, of incubation and were inhibited by 100 μM indomethacin. Under the above ideal conditions enterocyte microsomes yielded the following products expressed as pmole/mg protein/20 min, incubation: PGF 98±7, PGE2 48±9, PGD2 28±7, TxB2 40±5, 6 Keto PGF 15 ± 6.

Original languageEnglish (US)
Pages (from-to)609-624
Number of pages16
JournalProstaglandins
Volume31
Issue number4
DOIs
StatePublished - Apr 1986

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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