Protection against exotoxin A (ETA) and Pseudomonas aeruginosa infection in mice with ETA-specific antipeptide antibodies

Haissam S. El-Zaim, Ashok Chopra, Johnny Peterson, Michael L. Vasil, John P. Heggers

Research output: Contribution to journalArticle

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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes serious and sometimes fatal infections in the compromised host, especially in patients with major trauma or thermal injuries. Exotoxin A (ETA) is the major and most lethal virulence factor produced by this ubiquitous microorganism. In a recent study (H. S. Elzaim, A. K. Chopra, J. W. Peterson, R. Goodheart, and J.P. Heggers, Infect. Immun. 66:2170-2179, 1998), we identified two major epitopes, one within the translocation domain (amino acid [aa] residues 289 to 333) of ETA and another within the enzymatic domain (aa 610 to 638), by using a panel of antipeptide antibodies. Synthetic peptides representing these two epitopes induced ETA-specific antibodies which were able to abrogate the cytotoxic activity of ETA, as measured by incorporation of [3H]leucine into 3T3 fibroblasts. In the present study, these antibodies were tested for the ability to provide protection against ETA and infection with a toxin-producing strain of P. aeruginosa in a mouse model. Antibodies to either of the synthetic peptides conferred protection against ETA. Also, when used for immunization, both peptides induced active immunity to ETA in mice. Antibodies to the peptide representing a region within the enzymatic domain of ETA, in combination with the antibiotic amikacin, enhanced the survival of mice infected with a toxin-producing strain of P. aeruginosa. Thus, antipeptide antibodies specific for ETA might be paired with antibiotic treatment for passive immunization of patients suffering from P. aeruginosa infection.

Original languageEnglish (US)
Pages (from-to)5551-5554
Number of pages4
JournalInfection and Immunity
Volume66
Issue number11
StatePublished - 1998

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Pseudomonas Infections
Exotoxins
Pseudomonas aeruginosa
Antibodies
Peptides
Epitopes
Active Immunity
Anti-Bacterial Agents
Amino Acids
Passive Immunization
Amikacin
Wounds and Injuries
Virulence Factors
Infection
Leucine
Immunization
Fibroblasts
Hot Temperature

ASJC Scopus subject areas

  • Immunology

Cite this

Protection against exotoxin A (ETA) and Pseudomonas aeruginosa infection in mice with ETA-specific antipeptide antibodies. / El-Zaim, Haissam S.; Chopra, Ashok; Peterson, Johnny; Vasil, Michael L.; Heggers, John P.

In: Infection and Immunity, Vol. 66, No. 11, 1998, p. 5551-5554.

Research output: Contribution to journalArticle

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