Protection by and maintenance of CD4 effector memory and effector T cell subsets in persistent malaria infection

Michael M. Opata, Akanni Adededji Abdoul Ibitokou, Victor H. Carpio, Karis M. Marshall, Brian E. Dillon, Jordan C. Carl, Kyle D. Wilson, Christine M. Arcari, Robin Stephens

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Protection at the peak of Plasmodium chabaudi blood-stage malaria infection is provided by CD4 T cells. We have shown that an increase in Th1 cells also correlates with protection during the persistent phase of malaria; however, it is unclear how these T cells are maintained. Persistent malaria infection promotes protection and generates both effector T cells (Teff), and effector memory T cells (Tem). We have previously defined new CD4 Teff (IL-7Rα-) subsets from Early (TeffEarly, CD62LhiCD27+) to Late (TeffLate, CD62LloCD27-) activation states. Here, we tested these effector and memory T cell subsets for their ability to survive and protect in vivo. We found that both polyclonal and P. chabaudi Merozoite Surface Protein-1 (MSP-1)-specific B5 TCR transgenic Tem survive better than Teff. Surprisingly, as Tem are associated with antigen persistence, Tem survive well even after clearance of infection. As previously shown during T cell contraction, TeffEarly, which can generate Tem, also survive better than other Teff subsets in uninfected recipients. Two other Tem survival mechanisms identified here are that low-level chronic infection promotes Tem both by driving their proliferation, and by programming production of Tem from Tcm. Protective CD4 T cell phenotypes have not been precisely determined in malaria, or other persistent infections. Therefore, we tested purified memory (Tmem) and Teff subsets in protection from peak pathology and parasitemia in immunocompromised recipient mice. Strikingly, among Tmem (IL-7Rαhi) subsets, only TemLate (CD62LloCD27-) reduced peak parasitemia (19%), though the dominant memory subset is TemEarly, which is not protective. In contrast, all Teff subsets reduced peak parasitemia by more than half, and mature Teff can generate Tem, though less. In summary, we have elucidated four mechanisms of Tem maintenance, and identified two long-lived T cell subsets (TemLate, TeffEarly) that may represent correlates of protection or a target for longer-lived vaccine-induced protection against malaria blood-stages.

Original languageEnglish (US)
Pages (from-to)e1006960
JournalPLoS Pathogens
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2018

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Eragrostis
T-Lymphocyte Subsets
Malaria
Maintenance
T-Lymphocytes
Infection
Parasitemia
Plasmodium chabaudi
Merozoite Surface Protein 1
Th1 Cells
Aptitude
Vaccines
Pathology
Phenotype
Antigens

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Protection by and maintenance of CD4 effector memory and effector T cell subsets in persistent malaria infection. / Opata, Michael M.; Ibitokou, Akanni Adededji Abdoul; Carpio, Victor H.; Marshall, Karis M.; Dillon, Brian E.; Carl, Jordan C.; Wilson, Kyle D.; Arcari, Christine M.; Stephens, Robin.

In: PLoS Pathogens, Vol. 14, No. 4, 01.04.2018, p. e1006960.

Research output: Contribution to journalArticle

Opata, MM, Ibitokou, AAA, Carpio, VH, Marshall, KM, Dillon, BE, Carl, JC, Wilson, KD, Arcari, CM & Stephens, R 2018, 'Protection by and maintenance of CD4 effector memory and effector T cell subsets in persistent malaria infection', PLoS Pathogens, vol. 14, no. 4, pp. e1006960. https://doi.org/10.1371/journal.ppat.1006960
Opata, Michael M. ; Ibitokou, Akanni Adededji Abdoul ; Carpio, Victor H. ; Marshall, Karis M. ; Dillon, Brian E. ; Carl, Jordan C. ; Wilson, Kyle D. ; Arcari, Christine M. ; Stephens, Robin. / Protection by and maintenance of CD4 effector memory and effector T cell subsets in persistent malaria infection. In: PLoS Pathogens. 2018 ; Vol. 14, No. 4. pp. e1006960.
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AU - Ibitokou, Akanni Adededji Abdoul

AU - Carpio, Victor H.

AU - Marshall, Karis M.

AU - Dillon, Brian E.

AU - Carl, Jordan C.

AU - Wilson, Kyle D.

AU - Arcari, Christine M.

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