Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model

Sunhee Lee; Bo Young Jeon; Svetoslav Bardarov; Mei Chen; Sheldon L. Morris; William R. Jacobs

doi:10.1128/IAI.00531-06

Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model

Sunhee Lee, Bo Young Jeon, Svetoslav Bardarov, Mei Chen, Sheldon L. Morris, William R. Jacobs

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We generated four individual glutamine synthetase (GS) mutants (ΔglnA1, ΔglnA2, ΔglnA3, and ΔglnA4) and one triple mutant (ΔglnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the ΔglnA1EA2 and ΔglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.

Original language	English (US)
Pages (from-to)	6491-6495
Number of pages	5
Journal	Infection and immunity
Volume	74
Issue number	11
DOIs	https://doi.org/10.1128/IAI.00531-06
State	Published - Nov 2006
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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AU - Jeon, Bo Young

AU - Bardarov, Svetoslav

AU - Chen, Mei

AU - Morris, Sheldon L.

AU - Jacobs, William R.

PY - 2006/11

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AB - We generated four individual glutamine synthetase (GS) mutants (ΔglnA1, ΔglnA2, ΔglnA3, and ΔglnA4) and one triple mutant (ΔglnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the ΔglnA1EA2 and ΔglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.

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Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model

Abstract

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