Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model

Sunhee Lee, Bo Young Jeon, Svetoslav Bardarov, Mei Chen, Sheldon L. Morris, William R. Jacobs

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We generated four individual glutamine synthetase (GS) mutants (ΔglnA1, ΔglnA2, ΔglnA3, and ΔglnA4) and one triple mutant (ΔglnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the ΔglnA1EA2 and ΔglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.

Original languageEnglish (US)
Pages (from-to)6491-6495
Number of pages5
JournalInfection and immunity
Volume74
Issue number11
DOIs
StatePublished - Nov 1 2006
Externally publishedYes

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Glutamate-Ammonia Ligase
Mycobacterium bovis
Glutamine
Mycobacterium tuberculosis
Phenotype
Growth
Aerosols
Inbred C57BL Mouse
Immunization
Vaccination
Enzymes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model. / Lee, Sunhee; Jeon, Bo Young; Bardarov, Svetoslav; Chen, Mei; Morris, Sheldon L.; Jacobs, William R.

In: Infection and immunity, Vol. 74, No. 11, 01.11.2006, p. 6491-6495.

Research output: Contribution to journalArticle

Lee, Sunhee ; Jeon, Bo Young ; Bardarov, Svetoslav ; Chen, Mei ; Morris, Sheldon L. ; Jacobs, William R. / Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model. In: Infection and immunity. 2006 ; Vol. 74, No. 11. pp. 6491-6495.
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