Protection in mice passively immunized with serum from cynomolgus macaques and humans vaccinated with recombinant plague vaccine (rF1V)

Patricia Fellows, Jeffrey Adamovicz, Justin Hartings, Robert Sherwood, William Mega, Trevor Brasel, Ed Barr, Lou Holland, Winston Lin, Amanda Rom, William Blackwelder, Jessica Price, Stephen Morris, Doris Snow, Mary Kate Hart

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Passive transfer models were developed to evaluate the ability of antibodies generated in cynomolgus macaques and humans vaccinated with a recombinant plague vaccine (rF1V) to protect naïve Swiss Webster mice against pneumonic plague. Development of the passive transfer model is intended to support clinical and nonclinical development of the rF1V vaccine. To evaluate protection, unfractionated serum collected from rF1V vaccinated cynomolgus macaques and human volunteers with known antibody titers to rF1, rV and rF1V was transferred into naïve Swiss Webster mice via the intraperitoneal route. Results of these studies demonstrated that passive immunization protected mice from challenge or extended mean survival time and that the passive transfer assay can be used to evaluate the functional role of antibodies induced by rF1V vaccination in protection against aerosol exposure.

Original languageEnglish (US)
Pages (from-to)7748-7756
Number of pages9
JournalVaccine
Volume28
Issue number49
DOIs
StatePublished - Nov 16 2010

Keywords

  • Passive transfer
  • Pneumonic plague
  • RF1V vaccine
  • Yersinia pestis

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Fellows, P., Adamovicz, J., Hartings, J., Sherwood, R., Mega, W., Brasel, T., Barr, E., Holland, L., Lin, W., Rom, A., Blackwelder, W., Price, J., Morris, S., Snow, D., & Hart, M. K. (2010). Protection in mice passively immunized with serum from cynomolgus macaques and humans vaccinated with recombinant plague vaccine (rF1V). Vaccine, 28(49), 7748-7756. https://doi.org/10.1016/j.vaccine.2010.09.062