Protection of burn mice infected with herpesviruses by benzoylmesaconine (BEN) combined with IL-12

Fujio Suzuki, Makiko Kobayashi, David Herndon, R. B. Pollard

Research output: Contribution to journalArticle

Abstract

The majority of death in thermally injured patients is associated with infection. Herpesviruses have been identified as typical viral pathogens in bum patients. In the present study the effect of BEN combined with IL-12 treatments on the infection of herpes simplex virus type 1 (HSV) in thermally injured mice (TI-mice) was examined, because TI-mice become susceptible to the HSV infection based upon generating burn -associated CD8+ type 2 T cells which are influenced by these two substances in different mechanisms. BALB/c mice, subjected to a flame burn (3rd degree, 30% of total body surface area), were challenged i.p. with HSV (KOS strain). BEN, a non-toxic alkaloid extracted from Aconiti tuber, was supplied from Tsumura & Co., Tokyo, Japan. IL-12 was kindly provided from Genetics Institute, Cambridge, MA, and Hoffmann-La Roche, Inc., Nutley, NJ. TI-mice infected with HSV (4 × 103 PFU/kg) were treated orally with 0.1 μg/kg of BEN and/or 500 U/mouse of IL-12 i.p. IL-12 protected TI-mice infected with a lethal dose of HSV it was administered to mice before the infection. Mortalities in 80%, 90% or 90% of TI-mice infected with HSV were shown when they were treated with IL-12, BEN or saline (0.2 ml/mouse) one and 3 days after the infection, respectively. However, 80% of TI-mice survived when they were treated therapeutically in a combination with BEN and IL-12. These results suggest that synergistic antiviral effects are produced in TI-mice infected with HSV by a combined administration of BEN and IL-12.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

Herpesviridae
interleukin-12
Interleukin-12
Viruses
Human herpesvirus 1
Human Herpesvirus 1
mice
infection
Infection
T-cells
Pathogens
benzoylmesaconine
Alkaloids
Antiviral Agents
Tokyo
Body Surface Area
lethal dose
Virus Diseases
alkaloids
surface area

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Protection of burn mice infected with herpesviruses by benzoylmesaconine (BEN) combined with IL-12. / Suzuki, Fujio; Kobayashi, Makiko; Herndon, David; Pollard, R. B.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Suzuki, Fujio ; Kobayashi, Makiko ; Herndon, David ; Pollard, R. B. / Protection of burn mice infected with herpesviruses by benzoylmesaconine (BEN) combined with IL-12. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
@article{8df7d3b7363546859938ede077d51d11,
title = "Protection of burn mice infected with herpesviruses by benzoylmesaconine (BEN) combined with IL-12",
abstract = "The majority of death in thermally injured patients is associated with infection. Herpesviruses have been identified as typical viral pathogens in bum patients. In the present study the effect of BEN combined with IL-12 treatments on the infection of herpes simplex virus type 1 (HSV) in thermally injured mice (TI-mice) was examined, because TI-mice become susceptible to the HSV infection based upon generating burn -associated CD8+ type 2 T cells which are influenced by these two substances in different mechanisms. BALB/c mice, subjected to a flame burn (3rd degree, 30{\%} of total body surface area), were challenged i.p. with HSV (KOS strain). BEN, a non-toxic alkaloid extracted from Aconiti tuber, was supplied from Tsumura & Co., Tokyo, Japan. IL-12 was kindly provided from Genetics Institute, Cambridge, MA, and Hoffmann-La Roche, Inc., Nutley, NJ. TI-mice infected with HSV (4 × 103 PFU/kg) were treated orally with 0.1 μg/kg of BEN and/or 500 U/mouse of IL-12 i.p. IL-12 protected TI-mice infected with a lethal dose of HSV it was administered to mice before the infection. Mortalities in 80{\%}, 90{\%} or 90{\%} of TI-mice infected with HSV were shown when they were treated with IL-12, BEN or saline (0.2 ml/mouse) one and 3 days after the infection, respectively. However, 80{\%} of TI-mice survived when they were treated therapeutically in a combination with BEN and IL-12. These results suggest that synergistic antiviral effects are produced in TI-mice infected with HSV by a combined administration of BEN and IL-12.",
author = "Fujio Suzuki and Makiko Kobayashi and David Herndon and Pollard, {R. B.}",
year = "1996",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - Protection of burn mice infected with herpesviruses by benzoylmesaconine (BEN) combined with IL-12

AU - Suzuki, Fujio

AU - Kobayashi, Makiko

AU - Herndon, David

AU - Pollard, R. B.

PY - 1996

Y1 - 1996

N2 - The majority of death in thermally injured patients is associated with infection. Herpesviruses have been identified as typical viral pathogens in bum patients. In the present study the effect of BEN combined with IL-12 treatments on the infection of herpes simplex virus type 1 (HSV) in thermally injured mice (TI-mice) was examined, because TI-mice become susceptible to the HSV infection based upon generating burn -associated CD8+ type 2 T cells which are influenced by these two substances in different mechanisms. BALB/c mice, subjected to a flame burn (3rd degree, 30% of total body surface area), were challenged i.p. with HSV (KOS strain). BEN, a non-toxic alkaloid extracted from Aconiti tuber, was supplied from Tsumura & Co., Tokyo, Japan. IL-12 was kindly provided from Genetics Institute, Cambridge, MA, and Hoffmann-La Roche, Inc., Nutley, NJ. TI-mice infected with HSV (4 × 103 PFU/kg) were treated orally with 0.1 μg/kg of BEN and/or 500 U/mouse of IL-12 i.p. IL-12 protected TI-mice infected with a lethal dose of HSV it was administered to mice before the infection. Mortalities in 80%, 90% or 90% of TI-mice infected with HSV were shown when they were treated with IL-12, BEN or saline (0.2 ml/mouse) one and 3 days after the infection, respectively. However, 80% of TI-mice survived when they were treated therapeutically in a combination with BEN and IL-12. These results suggest that synergistic antiviral effects are produced in TI-mice infected with HSV by a combined administration of BEN and IL-12.

AB - The majority of death in thermally injured patients is associated with infection. Herpesviruses have been identified as typical viral pathogens in bum patients. In the present study the effect of BEN combined with IL-12 treatments on the infection of herpes simplex virus type 1 (HSV) in thermally injured mice (TI-mice) was examined, because TI-mice become susceptible to the HSV infection based upon generating burn -associated CD8+ type 2 T cells which are influenced by these two substances in different mechanisms. BALB/c mice, subjected to a flame burn (3rd degree, 30% of total body surface area), were challenged i.p. with HSV (KOS strain). BEN, a non-toxic alkaloid extracted from Aconiti tuber, was supplied from Tsumura & Co., Tokyo, Japan. IL-12 was kindly provided from Genetics Institute, Cambridge, MA, and Hoffmann-La Roche, Inc., Nutley, NJ. TI-mice infected with HSV (4 × 103 PFU/kg) were treated orally with 0.1 μg/kg of BEN and/or 500 U/mouse of IL-12 i.p. IL-12 protected TI-mice infected with a lethal dose of HSV it was administered to mice before the infection. Mortalities in 80%, 90% or 90% of TI-mice infected with HSV were shown when they were treated with IL-12, BEN or saline (0.2 ml/mouse) one and 3 days after the infection, respectively. However, 80% of TI-mice survived when they were treated therapeutically in a combination with BEN and IL-12. These results suggest that synergistic antiviral effects are produced in TI-mice infected with HSV by a combined administration of BEN and IL-12.

UR - http://www.scopus.com/inward/record.url?scp=33749097497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749097497&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -