Protection of non-human primates against glanders with a gold nanoparticle glycoconjugate vaccine

Alfredo G. Torres, Anthony E. Gregory, Christopher L. Hatcher, Heather Vinet-Oliphant, Lisa A. Morici, Richard W. Titball, Chad J. Roy

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The Gram-negative Burkholderia mallei is a zoonotic pathogen and the causative agent of glanders disease. Because the bacteria maintain the potential to be used as a biothreat agent, vaccine strategies are required for human glanders prophylaxis. A rhesus macaque (Macaca mulatta) model of pneumonic (inhalational) glanders was established and the protective properties of a nanoparticle glycoconjugate vaccine composed of Burkholderia thailandensis LPS conjugated to FliC was evaluated. An aerosol challenge dose of ~1×104CFU B. mallei produced mortality in 50% of naïve animals (n=2/4), 2-3 days post-exposure. Although survival benefit was not observed by vaccination with a glycoconjugate glanders vaccine (p=0.42), serum LPS-specific IgG titers were significantly higher on day 80 in 3 vaccinated animals who survived compared with 3 vaccinated animals who died. Furthermore, B. mallei was isolated from multiple organs of both non-vaccinated survivors, but not from any organs of 3 vaccinated survivors at 30 days post-challenge. Taken together, this is the first time a candidate vaccine has been evaluated in a non-human primate aerosol model of glanders and represents the initial step for consideration in pre-clinical studies.

Original languageEnglish (US)
Pages (from-to)686-692
Number of pages7
JournalVaccine
Volume33
Issue number5
DOIs
StatePublished - Jan 29 2015

Keywords

  • Aerosol
  • Burkholderia mallei
  • Glanders
  • Glycoconjugate
  • Macaques
  • Nanoparticles

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Torres, A. G., Gregory, A. E., Hatcher, C. L., Vinet-Oliphant, H., Morici, L. A., Titball, R. W., & Roy, C. J. (2015). Protection of non-human primates against glanders with a gold nanoparticle glycoconjugate vaccine. Vaccine, 33(5), 686-692. https://doi.org/10.1016/j.vaccine.2014.11.057