Protection of retinal pigment epithelial cells from oxidative damage by oltipraz, a cancer chemopreventive agent

Kasey C. Nelson, Jeffrey S. Armstrong, Siobhan Moriarty, Jiyang Cai, Mei Whey H Wu, Paul Sternberg, Dean P. Jones

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Objective. To determine whether oltipraz (4-methyl-5-pyrazinyl-3H-1,2-dithiole-3-thione) protects against oxidative injury in cultured human retinal pigment epithelial (hRPE) cells. Methods. Primary cultured hRPE cells were incubated with various concentrations of oltipraz followed by treatment with the chemical oxidant tert-butylhydroperoxide (tBH). Cell viability was assessed by release of lactate dehydrogenase (LDH) and cleavage of WST-1. Intracellular and mitochondrial levels of glutathione (GSH) were measured by HPLC. Glutathione S-transferase (GST), NADPH-quinone reductase (NQR), and glutathione peroxidase (GPx) were measured by specific enzyme activity assays. Results. Treatment of hRPE cells with oltipraz inhibited tBH-induced cell death in a concentration-dependent manner with significant inhibition at 50 μM. Olitpraz (50 μM) increased GSH levels in hRPE cells by approximately 18% and in hRPE mitochondrial fractions by approximately 50% after 24 hours of exposure. Treatment with oltipraz increased GST and NQR activities by approximately 21% and 11%, respectively. Conclusions. Oltipraz protects hRPE cells against tBH induced injury. The mechanism of protection is likely to include increased cellular and mitochondrial GSH levels and induction of detoxification enzymes, including GST and NQR. Dietary supplementation with oltipraz or other dithiolethiones may help protect the hRPE against oxidant induced injury.

Original languageEnglish (US)
Pages (from-to)3550-3554
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume43
Issue number11
StatePublished - Nov 2002
Externally publishedYes

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Retinal Pigments
Epithelial Cells
NAD(P)H Dehydrogenase (Quinone)
tert-Butylhydroperoxide
Glutathione Transferase
NADP
Neoplasms
Oxidants
Wounds and Injuries
Enzyme Induction
Enzyme Assays
Glutathione Peroxidase
Dietary Supplements
oltipraz
L-Lactate Dehydrogenase
Glutathione
Cell Survival
Cell Death
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Nelson, K. C., Armstrong, J. S., Moriarty, S., Cai, J., Wu, M. W. H., Sternberg, P., & Jones, D. P. (2002). Protection of retinal pigment epithelial cells from oxidative damage by oltipraz, a cancer chemopreventive agent. Investigative Ophthalmology and Visual Science, 43(11), 3550-3554.

Protection of retinal pigment epithelial cells from oxidative damage by oltipraz, a cancer chemopreventive agent. / Nelson, Kasey C.; Armstrong, Jeffrey S.; Moriarty, Siobhan; Cai, Jiyang; Wu, Mei Whey H; Sternberg, Paul; Jones, Dean P.

In: Investigative Ophthalmology and Visual Science, Vol. 43, No. 11, 11.2002, p. 3550-3554.

Research output: Contribution to journalArticle

Nelson, KC, Armstrong, JS, Moriarty, S, Cai, J, Wu, MWH, Sternberg, P & Jones, DP 2002, 'Protection of retinal pigment epithelial cells from oxidative damage by oltipraz, a cancer chemopreventive agent', Investigative Ophthalmology and Visual Science, vol. 43, no. 11, pp. 3550-3554.
Nelson, Kasey C. ; Armstrong, Jeffrey S. ; Moriarty, Siobhan ; Cai, Jiyang ; Wu, Mei Whey H ; Sternberg, Paul ; Jones, Dean P. / Protection of retinal pigment epithelial cells from oxidative damage by oltipraz, a cancer chemopreventive agent. In: Investigative Ophthalmology and Visual Science. 2002 ; Vol. 43, No. 11. pp. 3550-3554.
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abstract = "Objective. To determine whether oltipraz (4-methyl-5-pyrazinyl-3H-1,2-dithiole-3-thione) protects against oxidative injury in cultured human retinal pigment epithelial (hRPE) cells. Methods. Primary cultured hRPE cells were incubated with various concentrations of oltipraz followed by treatment with the chemical oxidant tert-butylhydroperoxide (tBH). Cell viability was assessed by release of lactate dehydrogenase (LDH) and cleavage of WST-1. Intracellular and mitochondrial levels of glutathione (GSH) were measured by HPLC. Glutathione S-transferase (GST), NADPH-quinone reductase (NQR), and glutathione peroxidase (GPx) were measured by specific enzyme activity assays. Results. Treatment of hRPE cells with oltipraz inhibited tBH-induced cell death in a concentration-dependent manner with significant inhibition at 50 μM. Olitpraz (50 μM) increased GSH levels in hRPE cells by approximately 18{\%} and in hRPE mitochondrial fractions by approximately 50{\%} after 24 hours of exposure. Treatment with oltipraz increased GST and NQR activities by approximately 21{\%} and 11{\%}, respectively. Conclusions. Oltipraz protects hRPE cells against tBH induced injury. The mechanism of protection is likely to include increased cellular and mitochondrial GSH levels and induction of detoxification enzymes, including GST and NQR. Dietary supplementation with oltipraz or other dithiolethiones may help protect the hRPE against oxidant induced injury.",
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N2 - Objective. To determine whether oltipraz (4-methyl-5-pyrazinyl-3H-1,2-dithiole-3-thione) protects against oxidative injury in cultured human retinal pigment epithelial (hRPE) cells. Methods. Primary cultured hRPE cells were incubated with various concentrations of oltipraz followed by treatment with the chemical oxidant tert-butylhydroperoxide (tBH). Cell viability was assessed by release of lactate dehydrogenase (LDH) and cleavage of WST-1. Intracellular and mitochondrial levels of glutathione (GSH) were measured by HPLC. Glutathione S-transferase (GST), NADPH-quinone reductase (NQR), and glutathione peroxidase (GPx) were measured by specific enzyme activity assays. Results. Treatment of hRPE cells with oltipraz inhibited tBH-induced cell death in a concentration-dependent manner with significant inhibition at 50 μM. Olitpraz (50 μM) increased GSH levels in hRPE cells by approximately 18% and in hRPE mitochondrial fractions by approximately 50% after 24 hours of exposure. Treatment with oltipraz increased GST and NQR activities by approximately 21% and 11%, respectively. Conclusions. Oltipraz protects hRPE cells against tBH induced injury. The mechanism of protection is likely to include increased cellular and mitochondrial GSH levels and induction of detoxification enzymes, including GST and NQR. Dietary supplementation with oltipraz or other dithiolethiones may help protect the hRPE against oxidant induced injury.

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