Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats

A comparison with n-acetylcysteine

Akbar Ahmad, Stefania Mondello, Rosanna Di Paola, Emanuela Mazzon, Emanuela Esposito, Maria Antonietta Catania, Domenico Italiano, Patrizia Mondello, Carmela Aloisi, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The aim of this study was to investigate the effects of apocynin, a NADPH (nicotinamide adenine dinucleotide phosphate)-oxidase inhibitor, in diabetic rats with nephropathy induced by contrast medium (CIN). Diabetes was induced in male Wistar rats by a single dose of streptozotocin (60 mg/kg i.v.). Animals were then divided into the following groups: 1) control group (diabetic rats treated i.v. with saline solution); 2) iomeprol group (iomeprol at 10 ml/kg was injected i.v. 30 min after saline administration); 3) apocynin group (identical to the iomeprol group, except for pre-treatment with apocynin 5 mg/kg i.v., 30 min before iomeprol injection) and 4) N-acetylcysteine group (NAC) (same as iomeprol group, except for the treatment with NAC 20 mg/kg i.v. 30 min before iomeprol injection). CIN in animals were assessed 24 h after administration of iomeprol. Apocynin significantly attenuates the impaired glomerular function, concentration of Na +, K +, alpha glutathione S-transferase levels in urine and neutrophil gelatinase-associated lipocalin levels in plasma caused by iomeprol. In kidney, immunohistochemical analysis of some inflammatory mediators, such as nitrotyrosine, poly-ADP-ribosyl polymerase, tumor necrosis factor-α, interleukin-1β as well as apoptosis (evaluated as terminal deoxynucleotidyltransferase-mediated UTP end labeling assay) revealed positive staining in tissue obtained from iomeprol group. These parameters were markedly reduced in animals treated with apocynin. Similarly, these parameters were also markedly modified by NAC pre-treatment. Here, we have shown that apocynin attenuates the degree of iomeprol-induced nephropathy in diabetic rats.

Original languageEnglish (US)
Pages (from-to)397-406
Number of pages10
JournalEuropean Journal of Pharmacology
Volume674
Issue number2-3
DOIs
StatePublished - Jan 14 2012
Externally publishedYes

Fingerprint

Acetylcysteine
Diabetic Nephropathies
NADP
Oxidoreductases
iomeprol
acetovanillone
Uridine Triphosphate
Injections
DNA Nucleotidylexotransferase
Streptozocin
Interleukin-1
Sodium Chloride
Adenosine Diphosphate
Contrast Media
Wistar Rats
Therapeutics
Tumor Necrosis Factor-alpha
Urine
Apoptosis
Staining and Labeling

Keywords

  • Apocynin
  • Contrast induced nephropathy
  • NADPH-oxidase

ASJC Scopus subject areas

  • Pharmacology

Cite this

Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats : A comparison with n-acetylcysteine. / Ahmad, Akbar; Mondello, Stefania; Di Paola, Rosanna; Mazzon, Emanuela; Esposito, Emanuela; Catania, Maria Antonietta; Italiano, Domenico; Mondello, Patrizia; Aloisi, Carmela; Cuzzocrea, Salvatore.

In: European Journal of Pharmacology, Vol. 674, No. 2-3, 14.01.2012, p. 397-406.

Research output: Contribution to journalArticle

Ahmad, A, Mondello, S, Di Paola, R, Mazzon, E, Esposito, E, Catania, MA, Italiano, D, Mondello, P, Aloisi, C & Cuzzocrea, S 2012, 'Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats: A comparison with n-acetylcysteine', European Journal of Pharmacology, vol. 674, no. 2-3, pp. 397-406. https://doi.org/10.1016/j.ejphar.2011.10.041
Ahmad, Akbar ; Mondello, Stefania ; Di Paola, Rosanna ; Mazzon, Emanuela ; Esposito, Emanuela ; Catania, Maria Antonietta ; Italiano, Domenico ; Mondello, Patrizia ; Aloisi, Carmela ; Cuzzocrea, Salvatore. / Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats : A comparison with n-acetylcysteine. In: European Journal of Pharmacology. 2012 ; Vol. 674, No. 2-3. pp. 397-406.
@article{f57047e2f5f449cf9af61ec203a25fb5,
title = "Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats: A comparison with n-acetylcysteine",
abstract = "The aim of this study was to investigate the effects of apocynin, a NADPH (nicotinamide adenine dinucleotide phosphate)-oxidase inhibitor, in diabetic rats with nephropathy induced by contrast medium (CIN). Diabetes was induced in male Wistar rats by a single dose of streptozotocin (60 mg/kg i.v.). Animals were then divided into the following groups: 1) control group (diabetic rats treated i.v. with saline solution); 2) iomeprol group (iomeprol at 10 ml/kg was injected i.v. 30 min after saline administration); 3) apocynin group (identical to the iomeprol group, except for pre-treatment with apocynin 5 mg/kg i.v., 30 min before iomeprol injection) and 4) N-acetylcysteine group (NAC) (same as iomeprol group, except for the treatment with NAC 20 mg/kg i.v. 30 min before iomeprol injection). CIN in animals were assessed 24 h after administration of iomeprol. Apocynin significantly attenuates the impaired glomerular function, concentration of Na +, K +, alpha glutathione S-transferase levels in urine and neutrophil gelatinase-associated lipocalin levels in plasma caused by iomeprol. In kidney, immunohistochemical analysis of some inflammatory mediators, such as nitrotyrosine, poly-ADP-ribosyl polymerase, tumor necrosis factor-α, interleukin-1β as well as apoptosis (evaluated as terminal deoxynucleotidyltransferase-mediated UTP end labeling assay) revealed positive staining in tissue obtained from iomeprol group. These parameters were markedly reduced in animals treated with apocynin. Similarly, these parameters were also markedly modified by NAC pre-treatment. Here, we have shown that apocynin attenuates the degree of iomeprol-induced nephropathy in diabetic rats.",
keywords = "Apocynin, Contrast induced nephropathy, NADPH-oxidase",
author = "Akbar Ahmad and Stefania Mondello and {Di Paola}, Rosanna and Emanuela Mazzon and Emanuela Esposito and Catania, {Maria Antonietta} and Domenico Italiano and Patrizia Mondello and Carmela Aloisi and Salvatore Cuzzocrea",
year = "2012",
month = "1",
day = "14",
doi = "10.1016/j.ejphar.2011.10.041",
language = "English (US)",
volume = "674",
pages = "397--406",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats

T2 - A comparison with n-acetylcysteine

AU - Ahmad, Akbar

AU - Mondello, Stefania

AU - Di Paola, Rosanna

AU - Mazzon, Emanuela

AU - Esposito, Emanuela

AU - Catania, Maria Antonietta

AU - Italiano, Domenico

AU - Mondello, Patrizia

AU - Aloisi, Carmela

AU - Cuzzocrea, Salvatore

PY - 2012/1/14

Y1 - 2012/1/14

N2 - The aim of this study was to investigate the effects of apocynin, a NADPH (nicotinamide adenine dinucleotide phosphate)-oxidase inhibitor, in diabetic rats with nephropathy induced by contrast medium (CIN). Diabetes was induced in male Wistar rats by a single dose of streptozotocin (60 mg/kg i.v.). Animals were then divided into the following groups: 1) control group (diabetic rats treated i.v. with saline solution); 2) iomeprol group (iomeprol at 10 ml/kg was injected i.v. 30 min after saline administration); 3) apocynin group (identical to the iomeprol group, except for pre-treatment with apocynin 5 mg/kg i.v., 30 min before iomeprol injection) and 4) N-acetylcysteine group (NAC) (same as iomeprol group, except for the treatment with NAC 20 mg/kg i.v. 30 min before iomeprol injection). CIN in animals were assessed 24 h after administration of iomeprol. Apocynin significantly attenuates the impaired glomerular function, concentration of Na +, K +, alpha glutathione S-transferase levels in urine and neutrophil gelatinase-associated lipocalin levels in plasma caused by iomeprol. In kidney, immunohistochemical analysis of some inflammatory mediators, such as nitrotyrosine, poly-ADP-ribosyl polymerase, tumor necrosis factor-α, interleukin-1β as well as apoptosis (evaluated as terminal deoxynucleotidyltransferase-mediated UTP end labeling assay) revealed positive staining in tissue obtained from iomeprol group. These parameters were markedly reduced in animals treated with apocynin. Similarly, these parameters were also markedly modified by NAC pre-treatment. Here, we have shown that apocynin attenuates the degree of iomeprol-induced nephropathy in diabetic rats.

AB - The aim of this study was to investigate the effects of apocynin, a NADPH (nicotinamide adenine dinucleotide phosphate)-oxidase inhibitor, in diabetic rats with nephropathy induced by contrast medium (CIN). Diabetes was induced in male Wistar rats by a single dose of streptozotocin (60 mg/kg i.v.). Animals were then divided into the following groups: 1) control group (diabetic rats treated i.v. with saline solution); 2) iomeprol group (iomeprol at 10 ml/kg was injected i.v. 30 min after saline administration); 3) apocynin group (identical to the iomeprol group, except for pre-treatment with apocynin 5 mg/kg i.v., 30 min before iomeprol injection) and 4) N-acetylcysteine group (NAC) (same as iomeprol group, except for the treatment with NAC 20 mg/kg i.v. 30 min before iomeprol injection). CIN in animals were assessed 24 h after administration of iomeprol. Apocynin significantly attenuates the impaired glomerular function, concentration of Na +, K +, alpha glutathione S-transferase levels in urine and neutrophil gelatinase-associated lipocalin levels in plasma caused by iomeprol. In kidney, immunohistochemical analysis of some inflammatory mediators, such as nitrotyrosine, poly-ADP-ribosyl polymerase, tumor necrosis factor-α, interleukin-1β as well as apoptosis (evaluated as terminal deoxynucleotidyltransferase-mediated UTP end labeling assay) revealed positive staining in tissue obtained from iomeprol group. These parameters were markedly reduced in animals treated with apocynin. Similarly, these parameters were also markedly modified by NAC pre-treatment. Here, we have shown that apocynin attenuates the degree of iomeprol-induced nephropathy in diabetic rats.

KW - Apocynin

KW - Contrast induced nephropathy

KW - NADPH-oxidase

UR - http://www.scopus.com/inward/record.url?scp=84855555390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855555390&partnerID=8YFLogxK

U2 - 10.1016/j.ejphar.2011.10.041

DO - 10.1016/j.ejphar.2011.10.041

M3 - Article

VL - 674

SP - 397

EP - 406

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2-3

ER -