Protective effect of hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation

Aimalohi Esechie, Levente Kiss, Gabor Olah, Eszter M. Horváth, Hal Hawkins, Csaba Szabo, Daniel L. Traber

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Acute lung injury results in a severe inflammatory response, which leads to priming and activation of leucocytes, release of reactive oxygen and reactive nitrogen species, destruction of pulmonary endothelium, extravasation of protein-rich fluid into the interstitium and formation of oedema. Recently, H2S (hydrogen sulfide) has been shown to decrease the synthesis of pro-inflammatory cytokines, reduce leucocyte adherence to the endothelium and subsequent diapedesis of these cells from the microvasculature in in vivo studies, and to protect cells in culture from oxidative injury. In the present study, we hypothesized that a parenteral formulation of H2S would reduce the lung injury induced by burn and smoke inhalation in a novel murine model. H2S post-treatment significantly decreased mortality and increased median survival in mice. H2S also inhibited IL (interleukin)-1β levels and significantly increased the concentration of the anti-inflammatory cytokine IL-10 in lung tissue. Additionally, H 2S administration attenuated protein oxidation following injury and improved the histological condition of the lung. In conclusion, these results suggest that H2S exerts protective effects in acute lung injury, at least in part through the activation of anti-inflammatory and antioxidant pathways.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalClinical Science
Volume115
Issue number3-4
DOIs
StatePublished - Aug 2008

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Inhalation Burns
Hydrogen Sulfide
Acute Lung Injury
Smoke
Lung
Endothelium
Leukocytes
Anti-Inflammatory Agents
Cytokines
Transendothelial and Transepithelial Migration
Reactive Nitrogen Species
Wounds and Injuries
Lung Injury
Microvessels
Interleukin-1
Interleukin-10
Edema
Proteins
Cell Culture Techniques
Antioxidants

Keywords

  • Acute lung injury
  • Burn and smoke inhalation
  • Hydrogen sulfide
  • Inflammation
  • Interleukin-10
  • Protein carbonylation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Protective effect of hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation. / Esechie, Aimalohi; Kiss, Levente; Olah, Gabor; Horváth, Eszter M.; Hawkins, Hal; Szabo, Csaba; Traber, Daniel L.

In: Clinical Science, Vol. 115, No. 3-4, 08.2008, p. 91-97.

Research output: Contribution to journalArticle

Esechie, Aimalohi ; Kiss, Levente ; Olah, Gabor ; Horváth, Eszter M. ; Hawkins, Hal ; Szabo, Csaba ; Traber, Daniel L. / Protective effect of hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation. In: Clinical Science. 2008 ; Vol. 115, No. 3-4. pp. 91-97.
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