TY - JOUR
T1 - Protective Effect of N-Acetyl Chitohexaose on Listeria monocytogenes Infection in Mice
AU - Tokoro, Akio
AU - Kobayashi, Makiko
AU - Tatewaki, Nobuhiko
AU - Mikami, Takeshi
AU - Suzuki, Ko
AU - Okawa, Yoshio
AU - Suzuki, Shigeo
AU - Suzuki, Masuko
PY - 1989
Y1 - 1989
N2 - A water-soluble oligosaccharide, N-acetyl chitohexaose (NACOS-6) was able to enhance the protecting effect of BALB/c male mice against Listeria mono-cytogenes infection, when administered intraperitoneally 24 hr before the challenge with this microbe. Significant decrease in number of microbes within the peritoneal cavity, spleen, and liver from the mice of NACOS-6-administered group was not observed 1 day after the infection but 4 days after the infection. Administration of NACOS-6 enhanced the delayed-type hypersensitivity response against sheep red blood cells (SRBC) or heat-killed L. monocytogenes. Splenic T lymphocytes from mice administered NACOS-6 released macrophage activating factor (MAF). These results suggested that NACOS-6 was also able to elevate the function of cellular immunity. Macrophages treated with a combination of NACOS-6 and the culture supernatant of splenic T lymphocytes from mice administered NACOS-6, “NACOS-6 sup,” were found to exert a fairly strong growth-inhibitory effect on L. monocytogenes. Interferon-γ (IFN-γ) and interleukin 2 (IL-2) were able to enhance the growth-inhibitory effect on L. monocytogenes by the NACOS-6-treated macrophages.
AB - A water-soluble oligosaccharide, N-acetyl chitohexaose (NACOS-6) was able to enhance the protecting effect of BALB/c male mice against Listeria mono-cytogenes infection, when administered intraperitoneally 24 hr before the challenge with this microbe. Significant decrease in number of microbes within the peritoneal cavity, spleen, and liver from the mice of NACOS-6-administered group was not observed 1 day after the infection but 4 days after the infection. Administration of NACOS-6 enhanced the delayed-type hypersensitivity response against sheep red blood cells (SRBC) or heat-killed L. monocytogenes. Splenic T lymphocytes from mice administered NACOS-6 released macrophage activating factor (MAF). These results suggested that NACOS-6 was also able to elevate the function of cellular immunity. Macrophages treated with a combination of NACOS-6 and the culture supernatant of splenic T lymphocytes from mice administered NACOS-6, “NACOS-6 sup,” were found to exert a fairly strong growth-inhibitory effect on L. monocytogenes. Interferon-γ (IFN-γ) and interleukin 2 (IL-2) were able to enhance the growth-inhibitory effect on L. monocytogenes by the NACOS-6-treated macrophages.
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U2 - 10.1111/j.1348-0421.1989.tb01983.x
DO - 10.1111/j.1348-0421.1989.tb01983.x
M3 - Article
C2 - 2505022
AN - SCOPUS:0024362928
SN - 0385-5600
VL - 33
SP - 357
EP - 367
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 4
ER -