Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice

A. Tokoro, Makiko Kobayashi, N. Tatewaki, K. Suzuki, Y. Okawa, T. Mikami, S. Suzuki, M. Suzuki

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

A water-soluble oligosacchasride, N-acetyl chitohexaose (NACOS-6) was able to enhance the protecting effect of BALB/c male mice against Listeria monocytogenes infection, when administered intraperitoneally 24 hr before the challenge with this microbe. Significant decrease in number of microbes within the peritoneal cavity, spleen, and liver from the mice of NACOS-6-administered group was not observed 1 day after the infection but 4 days after the infection. Administration of NACOS-6 enhanced the delayed-type hypersensitivity response against sheep red blood cells (SRBC) or heat-killed L. monocytogenes. Splenic T lymphocytes from mice administered NACOS-6 released macrophage activating factor (MAF). These results suggested that NACOS-6 was also able to elevate the function of cellular immunity. Macrophages treated with a combination of NACOS-6 and the culture supernatant of splenic T lymphocytes from mice administered NACOS-6, 'NACOS-6 sup', were found to exert a fairly strong growth-inhibitory effect on L. monocytogenes. Interferon-γ (IFN-γ) and interleukin 2 (IL-2) were able to enhance the growth-inhibitory effect on L. monocytogenes by the NACOS-6-treated macrophages.

Original languageEnglish (US)
Pages (from-to)357-367
Number of pages11
JournalMicrobiology and Immunology
Volume33
Issue number4
StatePublished - 1989
Externally publishedYes

Fingerprint

Listeriosis
Listeria monocytogenes
Macrophage-Activating Factors
Macrophages
T-Lymphocytes
N-acetylchitohexaose
chitohexaose
Peritoneal Cavity
Delayed Hypersensitivity
Growth
Infection
Cellular Immunity
Interferons
Interleukin-2
Sheep
Spleen
Hot Temperature
Erythrocytes
Water

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Tokoro, A., Kobayashi, M., Tatewaki, N., Suzuki, K., Okawa, Y., Mikami, T., ... Suzuki, M. (1989). Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice. Microbiology and Immunology, 33(4), 357-367.

Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice. / Tokoro, A.; Kobayashi, Makiko; Tatewaki, N.; Suzuki, K.; Okawa, Y.; Mikami, T.; Suzuki, S.; Suzuki, M.

In: Microbiology and Immunology, Vol. 33, No. 4, 1989, p. 357-367.

Research output: Contribution to journalArticle

Tokoro, A, Kobayashi, M, Tatewaki, N, Suzuki, K, Okawa, Y, Mikami, T, Suzuki, S & Suzuki, M 1989, 'Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice', Microbiology and Immunology, vol. 33, no. 4, pp. 357-367.
Tokoro A, Kobayashi M, Tatewaki N, Suzuki K, Okawa Y, Mikami T et al. Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice. Microbiology and Immunology. 1989;33(4):357-367.
Tokoro, A. ; Kobayashi, Makiko ; Tatewaki, N. ; Suzuki, K. ; Okawa, Y. ; Mikami, T. ; Suzuki, S. ; Suzuki, M. / Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice. In: Microbiology and Immunology. 1989 ; Vol. 33, No. 4. pp. 357-367.
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