Protective effect of Shigyaku-to, a traditional Chinese herbal medicine, on the infection of herpes simplex virus type 1 (HSV-1) in mice

K. Ikemoto, T. Utsunomiya, M. A. Ball, M. Kobayashi, R. B. Pollard, F. Suzuki

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The antiviral activity of Shigyaku-to (TJS-109), a traditional Chinese herbal medicine, was investigated in mice infected with herpes simplex virus type 1 (HSV-1). TJS-109 is a combination of the medicinal plant extracts from Zingiberis siccatum rhizoma, Aconiti tuber and Glycyrrhizae radix in a specific proportion. Mice infected with a 10 LD50 dose of HSV-1 were treated with TJS-109 orally at doses of 1.25 to 20 mg/kg 2 days before, and 1 and 4 days after the infection. The treated groups had 80% (1.25 mg/kg), 40% (5 mg/kg) and 23% (20 mg/kg) mortality rates 25 days after the infection as compared with a 100% mortality rate in control mice treated with saline. When HSV-1 infected mice (recipients) received CD8+T cell fractions derived from spleens of mice treated with TJS-109 (donors), 70% of recipients survived, as compared with 0% survivors in the groups of mice treated with saline, B cell fractions, CD4+ T cell fractions or macrophage-enriched fractions prepared from the same donors. TJS-109 did not show any virucidal activities against HSV-1 or any virostatic activities on the growth of HSV-1 in Vero cells. These results suggest that TJS-109 protected mice exposed to lethal amounts of HSV-1 through the activation of CD8+ T cells.

Original languageEnglish (US)
Pages (from-to)456-460
Number of pages5
JournalExperientia
Volume50
Issue number5
DOIs
StatePublished - May 1 1994

Keywords

  • CD8 T cells
  • Traditional Chinese herbal medicine
  • antiviral effects
  • herpes simplex virus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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