Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

N. G. Saravia; M. H. Hazbón; Y. Osorio; L. Valderrama; J. Walker; C. Santrich; T. Cortázar; J. H. LeBowitz; B. L. Travi

doi:10.1016/j.vaccine.2004.07.044

Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

N. G. Saravia, M. H. Hazbón, Y. Osorio, L. Valderrama, J. Walker, C. Santrich, T. Cortázar, J. H. LeBowitz, B. L. Travi

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Paraflagellar rod proteins (PFR) are a potent immunogen against experimental Trypanosoma cruzi infection. PFR are highly conserved among kinetoplastid parasites. We therefore evaluated the immunogenicity of the Leishmania mexicana pfr-2 gene and protein product in the hamster model of American cutaneous leishmaniasis. Immunization with pfr-2 DNA-induced specific antibody, confirming immunogenicity. Subsequent challenge with 10,000 and 500 stationary phase L. mexicana promastigotes respectively, resulted in delayed appearance of lesions, and significant reduction in lesions post infection in male hamsters, yet exacerbated lesions in female hamsters. Immunization with recombinant PFR-2 protein (rPFR-2) prevented lesion development in female hamsters challenged with L. panamensis, but was ineffective against L. mexicana. Nevertheless, prime boost immunization of female hamsters with rPFR and pfr-2 DNA significantly reduced lesion size following challenge with 500 L. mexicana promastigotes, supporting the relevance of PFR-2 as a potential vaccine constituent.

Original language	English (US)
Pages (from-to)	984-995
Number of pages	12
Journal	Vaccine
Volume	23
Issue number	8
DOIs	https://doi.org/10.1016/j.vaccine.2004.07.044
State	Published - Jan 11 2005
Externally published	Yes

Keywords

DNA vaccine
Leishmania
Paraflagellar rod protein

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/j.vaccine.2004.07.044

Cite this

@article{ee7c7e85b8cd4848b5cfcca6c2dad619,

title = "Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana",

abstract = "Paraflagellar rod proteins (PFR) are a potent immunogen against experimental Trypanosoma cruzi infection. PFR are highly conserved among kinetoplastid parasites. We therefore evaluated the immunogenicity of the Leishmania mexicana pfr-2 gene and protein product in the hamster model of American cutaneous leishmaniasis. Immunization with pfr-2 DNA-induced specific antibody, confirming immunogenicity. Subsequent challenge with 10,000 and 500 stationary phase L. mexicana promastigotes respectively, resulted in delayed appearance of lesions, and significant reduction in lesions post infection in male hamsters, yet exacerbated lesions in female hamsters. Immunization with recombinant PFR-2 protein (rPFR-2) prevented lesion development in female hamsters challenged with L. panamensis, but was ineffective against L. mexicana. Nevertheless, prime boost immunization of female hamsters with rPFR and pfr-2 DNA significantly reduced lesion size following challenge with 500 L. mexicana promastigotes, supporting the relevance of PFR-2 as a potential vaccine constituent.",

keywords = "DNA vaccine, Leishmania, Paraflagellar rod protein",

author = "Saravia, {N. G.} and Hazb{\'o}n, {M. H.} and Y. Osorio and L. Valderrama and J. Walker and C. Santrich and T. Cort{\'a}zar and LeBowitz, {J. H.} and Travi, {B. L.}",

note = "Funding Information: This study was supported in part by grant ID 980269 UNDP/WORLD BANK/WHO Special Programme for Research and Training in Tropical Diseases (TDR), and COLCIENCIAS grant 2229-05-224-99, 2229-05-228-99 and Colciencias young investigator program CT #021-2000. The technical assistance of Diego Goyeneche in the in vitro analysis of the immune response in hamsters are gratefully acknowledged. We thank Andres Hurtado, Ph.D. for his initiative and role in the design of the DNA vaccine for the conduct of this study, and Mauricio Perez for his invaluable assistance in the statistical analysis of the data.",

year = "2005",

month = jan,

day = "11",

doi = "10.1016/j.vaccine.2004.07.044",

language = "English (US)",

volume = "23",

pages = "984--995",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "8",

}

TY - JOUR

T1 - Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

AU - Saravia, N. G.

AU - Hazbón, M. H.

AU - Osorio, Y.

AU - Valderrama, L.

AU - Walker, J.

AU - Santrich, C.

AU - Cortázar, T.

AU - LeBowitz, J. H.

AU - Travi, B. L.

N1 - Funding Information: This study was supported in part by grant ID 980269 UNDP/WORLD BANK/WHO Special Programme for Research and Training in Tropical Diseases (TDR), and COLCIENCIAS grant 2229-05-224-99, 2229-05-228-99 and Colciencias young investigator program CT #021-2000. The technical assistance of Diego Goyeneche in the in vitro analysis of the immune response in hamsters are gratefully acknowledged. We thank Andres Hurtado, Ph.D. for his initiative and role in the design of the DNA vaccine for the conduct of this study, and Mauricio Perez for his invaluable assistance in the statistical analysis of the data.

PY - 2005/1/11

Y1 - 2005/1/11

N2 - Paraflagellar rod proteins (PFR) are a potent immunogen against experimental Trypanosoma cruzi infection. PFR are highly conserved among kinetoplastid parasites. We therefore evaluated the immunogenicity of the Leishmania mexicana pfr-2 gene and protein product in the hamster model of American cutaneous leishmaniasis. Immunization with pfr-2 DNA-induced specific antibody, confirming immunogenicity. Subsequent challenge with 10,000 and 500 stationary phase L. mexicana promastigotes respectively, resulted in delayed appearance of lesions, and significant reduction in lesions post infection in male hamsters, yet exacerbated lesions in female hamsters. Immunization with recombinant PFR-2 protein (rPFR-2) prevented lesion development in female hamsters challenged with L. panamensis, but was ineffective against L. mexicana. Nevertheless, prime boost immunization of female hamsters with rPFR and pfr-2 DNA significantly reduced lesion size following challenge with 500 L. mexicana promastigotes, supporting the relevance of PFR-2 as a potential vaccine constituent.

AB - Paraflagellar rod proteins (PFR) are a potent immunogen against experimental Trypanosoma cruzi infection. PFR are highly conserved among kinetoplastid parasites. We therefore evaluated the immunogenicity of the Leishmania mexicana pfr-2 gene and protein product in the hamster model of American cutaneous leishmaniasis. Immunization with pfr-2 DNA-induced specific antibody, confirming immunogenicity. Subsequent challenge with 10,000 and 500 stationary phase L. mexicana promastigotes respectively, resulted in delayed appearance of lesions, and significant reduction in lesions post infection in male hamsters, yet exacerbated lesions in female hamsters. Immunization with recombinant PFR-2 protein (rPFR-2) prevented lesion development in female hamsters challenged with L. panamensis, but was ineffective against L. mexicana. Nevertheless, prime boost immunization of female hamsters with rPFR and pfr-2 DNA significantly reduced lesion size following challenge with 500 L. mexicana promastigotes, supporting the relevance of PFR-2 as a potential vaccine constituent.

KW - DNA vaccine

KW - Leishmania

KW - Paraflagellar rod protein

UR - http://www.scopus.com/inward/record.url?scp=11144296349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144296349&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2004.07.044

DO - 10.1016/j.vaccine.2004.07.044

M3 - Article

C2 - 15620471

AN - SCOPUS:11144296349

SN - 0264-410X

VL - 23

SP - 984

EP - 995

JO - Vaccine

JF - Vaccine

IS - 8

ER -

Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this