Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

N. G. Saravia, M. H. Hazbón, Y. Osorio, L. Valderrama, J. Walker, C. Santrich, T. Cortázar, J. H. LeBowitz, Bruno Travi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Paraflagellar rod proteins (PFR) are a potent immunogen against experimental Trypanosoma cruzi infection. PFR are highly conserved among kinetoplastid parasites. We therefore evaluated the immunogenicity of the Leishmania mexicana pfr-2 gene and protein product in the hamster model of American cutaneous leishmaniasis. Immunization with pfr-2 DNA-induced specific antibody, confirming immunogenicity. Subsequent challenge with 10,000 and 500 stationary phase L. mexicana promastigotes respectively, resulted in delayed appearance of lesions, and significant reduction in lesions post infection in male hamsters, yet exacerbated lesions in female hamsters. Immunization with recombinant PFR-2 protein (rPFR-2) prevented lesion development in female hamsters challenged with L. panamensis, but was ineffective against L. mexicana. Nevertheless, prime boost immunization of female hamsters with rPFR and pfr-2 DNA significantly reduced lesion size following challenge with 500 L. mexicana promastigotes, supporting the relevance of PFR-2 as a potential vaccine constituent.

Original languageEnglish (US)
Pages (from-to)984-995
Number of pages12
JournalVaccine
Volume23
Issue number8
DOIs
StatePublished - Jan 11 2005
Externally publishedYes

Fingerprint

Leishmania mexicana
hamsters
lesions (animal)
Cricetinae
immune response
promastigotes
immunization
Immunization
Cutaneous Leishmaniasis
Proteins
proteins
cutaneous leishmaniasis
Trypanosoma cruzi
protein products
DNA
infection
Infection
vaccines
Parasites
antigens

Keywords

  • DNA vaccine
  • Leishmania
  • Paraflagellar rod protein

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Saravia, N. G., Hazbón, M. H., Osorio, Y., Valderrama, L., Walker, J., Santrich, C., ... Travi, B. (2005). Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana. Vaccine, 23(8), 984-995. https://doi.org/10.1016/j.vaccine.2004.07.044

Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana. / Saravia, N. G.; Hazbón, M. H.; Osorio, Y.; Valderrama, L.; Walker, J.; Santrich, C.; Cortázar, T.; LeBowitz, J. H.; Travi, Bruno.

In: Vaccine, Vol. 23, No. 8, 11.01.2005, p. 984-995.

Research output: Contribution to journalArticle

Saravia, NG, Hazbón, MH, Osorio, Y, Valderrama, L, Walker, J, Santrich, C, Cortázar, T, LeBowitz, JH & Travi, B 2005, 'Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana', Vaccine, vol. 23, no. 8, pp. 984-995. https://doi.org/10.1016/j.vaccine.2004.07.044
Saravia NG, Hazbón MH, Osorio Y, Valderrama L, Walker J, Santrich C et al. Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana. Vaccine. 2005 Jan 11;23(8):984-995. https://doi.org/10.1016/j.vaccine.2004.07.044
Saravia, N. G. ; Hazbón, M. H. ; Osorio, Y. ; Valderrama, L. ; Walker, J. ; Santrich, C. ; Cortázar, T. ; LeBowitz, J. H. ; Travi, Bruno. / Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana. In: Vaccine. 2005 ; Vol. 23, No. 8. pp. 984-995.
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