Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

N. G. Saravia, M. H. Hazbón, Y. Osorio, L. Valderrama, J. Walker, C. Santrich, T. Cortázar, J. H. LeBowitz, B. L. Travi

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Paraflagellar rod proteins (PFR) are a potent immunogen against experimental Trypanosoma cruzi infection. PFR are highly conserved among kinetoplastid parasites. We therefore evaluated the immunogenicity of the Leishmania mexicana pfr-2 gene and protein product in the hamster model of American cutaneous leishmaniasis. Immunization with pfr-2 DNA-induced specific antibody, confirming immunogenicity. Subsequent challenge with 10,000 and 500 stationary phase L. mexicana promastigotes respectively, resulted in delayed appearance of lesions, and significant reduction in lesions post infection in male hamsters, yet exacerbated lesions in female hamsters. Immunization with recombinant PFR-2 protein (rPFR-2) prevented lesion development in female hamsters challenged with L. panamensis, but was ineffective against L. mexicana. Nevertheless, prime boost immunization of female hamsters with rPFR and pfr-2 DNA significantly reduced lesion size following challenge with 500 L. mexicana promastigotes, supporting the relevance of PFR-2 as a potential vaccine constituent.

Original languageEnglish (US)
Pages (from-to)984-995
Number of pages12
JournalVaccine
Volume23
Issue number8
DOIs
StatePublished - Jan 11 2005
Externally publishedYes

Keywords

  • DNA vaccine
  • Leishmania
  • Paraflagellar rod protein

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Saravia, N. G., Hazbón, M. H., Osorio, Y., Valderrama, L., Walker, J., Santrich, C., Cortázar, T., LeBowitz, J. H., & Travi, B. L. (2005). Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana. Vaccine, 23(8), 984-995. https://doi.org/10.1016/j.vaccine.2004.07.044