Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics

Zbigniew Gugala, Sylwester Gogolewski

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The adsorption of proteins and growth and activity of primary rat osteoblasts cultured for 1, 2 and 3 weeks on nonporous and porous resorbable poly(L/DL-lactide) 80/20% membranes with defined surface characteristics were investigated. The growth and activity of cells were estimated from the measurements of DNA, alkaline phosphatase activity and the total amount of protein in the cell lysate. The cell morphology was assessed from scanning electron microscopy and rhodamine staining. The protein adsorption to the membrane surface was assessed from the amide I peak at 1640-1660cm-1 and the amide II peak at 1540-1560cm-1 in the attenuated total reflection infrared spectra. The relative amount of proteins adsorbed on the nonporous and porous membranes was comparable. The cells growing on the nonporous and porous membranes maintained the phenotype and revealed morphology typical for osteoblasts. The mineralized noduli were larger in size on the porous membranes. The number of cells, the amount of DNA, the alkaline phosphatase activity, and the total amount of protein increased with time of the experiment and were higher for the porous membranes than for the nonporous ones.

Original languageEnglish (US)
Pages (from-to)2341-2351
Number of pages11
JournalBiomaterials
Volume25
Issue number12
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Osteoblasts
Adsorption
Rats
Proteins
Membranes
Growth
Phosphatases
Amides
Alkaline Phosphatase
DNA
Rhodamines
Electron Scanning Microscopy
poly(lactide)
Cell Count
Cells
Staining and Labeling
Infrared radiation
Phenotype
Scanning electron microscopy
Experiments

Keywords

  • Cell culture
  • Osteoblasts
  • Polylactide
  • Protein adsorption
  • Resorbable membranes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Protein adsorption, attachment, growth and activity of primary rat osteoblasts on polylactide membranes with defined surface characteristics. / Gugala, Zbigniew; Gogolewski, Sylwester.

In: Biomaterials, Vol. 25, No. 12, 05.2004, p. 2341-2351.

Research output: Contribution to journalArticle

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