Protein blend ingestion following resistance exercise promotes human muscle protein synthesis

Paul T. Reidy, Dillon K. Walker, Jared M. Dickinson, David M. Gundermann, Micah J. Drummond, Kyle L. Timmerman, Christopher Fry, Michael S. Borack, Mark B. Cope, Ratna Mukherjea, Kristofer Jennings, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

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Abstract

High-quality proteins such as soy, whey, and casein are all capable of promoting muscle protein synthesis postexercise by activating the mammalian target of rapamycin (mTORC1) signaling pathway. We hypothesized that a protein blend of soy and dairy proteins would capitalize on the unique properties of each individual protein and allow for optimal delivery of amino acids to prolong the fractional synthetic rate (FSR) following resistance exercise (RE). In this double-blind, randomized, clinical trial, 19 young adults were studied before and after ingestion of ;19 g of protein blend (PB) or ;18 g whey protein (WP) consumed 1 h after high-intensity leg RE. We examined mixed-muscle protein FSR by stable isotopic methods and mTORC1 signaling with western blotting. Muscle biopsies from the vastus lateralis were collected at rest (before RE) and at 3 postexercise time points during an early (0-2 h) and late (2-4 h) postingestion period. WP ingestion resulted in higher and earlier amplitude of blood branched-chain amino acid (BCAA) concentrations. PB ingestion created a lower initial rise in blood BCAA but sustained elevated levels of blood amino acids later into recovery (P < 0.05). Postexercise FSR increased equivalently in both groups during the early period (WP, 0.078 6 0.009%; PB, 0.088 6 0.007%); however, FSR remained elevated only in the PB group during the late period (WP, 0.074 6 0.010%; PB, 0.087 6 0.003%) (P < 0.05). mTORC1 signaling similarly increased between groups, except for no increase in S6K1 phosphorylation in the WP group at 5 h postexercise (P < 0.05). We conclude that a soy-dairy PB ingested following exercise is capable of prolonging blood aminoacidemia, mTORC1 signaling, and protein synthesis in human skeletal muscle and is an effective postexercise nutritional supplement.

Original languageEnglish (US)
Pages (from-to)410-416
Number of pages7
JournalJournal of Nutrition
Volume143
Issue number4
DOIs
StatePublished - Apr 1 2013

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Muscle Proteins
Eating
Exercise
Proteins
Period Circadian Proteins
Branched Chain Amino Acids
Soybean Proteins
Amino Acids
Quadriceps Muscle
Sirolimus
Caseins
Young Adult
Leg
Skeletal Muscle
Randomized Controlled Trials
Western Blotting
Phosphorylation
Whey Proteins
Biopsy
Muscles

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Reidy, P. T., Walker, D. K., Dickinson, J. M., Gundermann, D. M., Drummond, M. J., Timmerman, K. L., ... Rasmussen, B. (2013). Protein blend ingestion following resistance exercise promotes human muscle protein synthesis. Journal of Nutrition, 143(4), 410-416. https://doi.org/10.3945/jn.112.168021

Protein blend ingestion following resistance exercise promotes human muscle protein synthesis. / Reidy, Paul T.; Walker, Dillon K.; Dickinson, Jared M.; Gundermann, David M.; Drummond, Micah J.; Timmerman, Kyle L.; Fry, Christopher; Borack, Michael S.; Cope, Mark B.; Mukherjea, Ratna; Jennings, Kristofer; Volpi, Elena; Rasmussen, Blake.

In: Journal of Nutrition, Vol. 143, No. 4, 01.04.2013, p. 410-416.

Research output: Contribution to journalArticle

Reidy, PT, Walker, DK, Dickinson, JM, Gundermann, DM, Drummond, MJ, Timmerman, KL, Fry, C, Borack, MS, Cope, MB, Mukherjea, R, Jennings, K, Volpi, E & Rasmussen, B 2013, 'Protein blend ingestion following resistance exercise promotes human muscle protein synthesis', Journal of Nutrition, vol. 143, no. 4, pp. 410-416. https://doi.org/10.3945/jn.112.168021
Reidy PT, Walker DK, Dickinson JM, Gundermann DM, Drummond MJ, Timmerman KL et al. Protein blend ingestion following resistance exercise promotes human muscle protein synthesis. Journal of Nutrition. 2013 Apr 1;143(4):410-416. https://doi.org/10.3945/jn.112.168021
Reidy, Paul T. ; Walker, Dillon K. ; Dickinson, Jared M. ; Gundermann, David M. ; Drummond, Micah J. ; Timmerman, Kyle L. ; Fry, Christopher ; Borack, Michael S. ; Cope, Mark B. ; Mukherjea, Ratna ; Jennings, Kristofer ; Volpi, Elena ; Rasmussen, Blake. / Protein blend ingestion following resistance exercise promotes human muscle protein synthesis. In: Journal of Nutrition. 2013 ; Vol. 143, No. 4. pp. 410-416.
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