Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei

J. Stenos, B. Ross, H. M. Feng, P. Crocquet-Valdes, David Walker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted fever group rickettsial species did not react with R. honei. A PCR product of the repeat region of the rOmp A gene from R. honei was amplified and calculated to contain 11 repeat units.

Original languageEnglish (US)
Pages (from-to)261-263
Number of pages3
JournalJournal of Clinical Microbiology
Volume35
Issue number1
StatePublished - 1997

Fingerprint

Rickettsia
Fever
Monoclonal Antibodies
vpr Genes
Proteins
Molecular Weight
Antigens
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei. / Stenos, J.; Ross, B.; Feng, H. M.; Crocquet-Valdes, P.; Walker, David.

In: Journal of Clinical Microbiology, Vol. 35, No. 1, 1997, p. 261-263.

Research output: Contribution to journalArticle

@article{e44b32a2e96f47c9a0a935e8017abcc5,
title = "Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei",
abstract = "Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted fever group rickettsial species did not react with R. honei. A PCR product of the repeat region of the rOmp A gene from R. honei was amplified and calculated to contain 11 repeat units.",
author = "J. Stenos and B. Ross and Feng, {H. M.} and P. Crocquet-Valdes and David Walker",
year = "1997",
language = "English (US)",
volume = "35",
pages = "261--263",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei

AU - Stenos, J.

AU - Ross, B.

AU - Feng, H. M.

AU - Crocquet-Valdes, P.

AU - Walker, David

PY - 1997

Y1 - 1997

N2 - Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted fever group rickettsial species did not react with R. honei. A PCR product of the repeat region of the rOmp A gene from R. honei was amplified and calculated to contain 11 repeat units.

AB - Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted fever group rickettsial species did not react with R. honei. A PCR product of the repeat region of the rOmp A gene from R. honei was amplified and calculated to contain 11 repeat units.

UR - http://www.scopus.com/inward/record.url?scp=0031059944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031059944&partnerID=8YFLogxK

M3 - Article

C2 - 8968920

AN - SCOPUS:0031059944

VL - 35

SP - 261

EP - 263

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -