Abstract
Lymph was collected from tail lymphatics of anesthetized rats, subcutaneous interstitial fluid was obtained by implanting nylon wicks, and tendon interstitial fluid was obtained by centrifugation of pieces of tendon. Spontaneous lymph flow rates averaged 70 nl.min-1.g skin-1. Protein concentrations and colloid osmotic pressure of sampled fluids differed significantly. Tail lymph had the highest protein concentration relative to plasma [lymph-to-plasma ratio 0.71 ± 0.03 (SE) n = 10], followed by wick fluid (0.62 ± 0.02, n = 9), with tendon fluid lowest (0.50 ± 0.03, n = 10). Albumin and immunolgobulin G (IgG) concentrations in samples of tail skin and tendon were assayed by rocket immunoelectrophoresis. Comparison of their distribution volumes at lymph or tendon fluid concentrations, respectively, with interstitial fluid volumes measured as 2-h 51Cr-ethylenediaminetetraacetic acid space minus 5-min 125I-albumin space indicated that 50-60% of the interstitial volume in these tissues is not available for distribution of albumin or IgG. Low lymph flow and high interstitial protein content or rat tail indicate a slow turnover of interstitial protein. This suggests that interstitial washout of protein plays a role in limiting edema only after a sustained or chronic increase in fluid filtration.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Heart and Circulatory Physiology |
| Volume | 16 |
| Issue number | 1 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physiology
Cite this
Protein concentration of lymph and interstitial fluid in the rat tail. / Aukland, K.; Kramer, George; Renkin, E. M.
In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 16, No. 1, 1984.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein concentration of lymph and interstitial fluid in the rat tail
AU - Aukland, K.
AU - Kramer, George
AU - Renkin, E. M.
PY - 1984
Y1 - 1984
N2 - Lymph was collected from tail lymphatics of anesthetized rats, subcutaneous interstitial fluid was obtained by implanting nylon wicks, and tendon interstitial fluid was obtained by centrifugation of pieces of tendon. Spontaneous lymph flow rates averaged 70 nl.min-1.g skin-1. Protein concentrations and colloid osmotic pressure of sampled fluids differed significantly. Tail lymph had the highest protein concentration relative to plasma [lymph-to-plasma ratio 0.71 ± 0.03 (SE) n = 10], followed by wick fluid (0.62 ± 0.02, n = 9), with tendon fluid lowest (0.50 ± 0.03, n = 10). Albumin and immunolgobulin G (IgG) concentrations in samples of tail skin and tendon were assayed by rocket immunoelectrophoresis. Comparison of their distribution volumes at lymph or tendon fluid concentrations, respectively, with interstitial fluid volumes measured as 2-h 51Cr-ethylenediaminetetraacetic acid space minus 5-min 125I-albumin space indicated that 50-60% of the interstitial volume in these tissues is not available for distribution of albumin or IgG. Low lymph flow and high interstitial protein content or rat tail indicate a slow turnover of interstitial protein. This suggests that interstitial washout of protein plays a role in limiting edema only after a sustained or chronic increase in fluid filtration.
AB - Lymph was collected from tail lymphatics of anesthetized rats, subcutaneous interstitial fluid was obtained by implanting nylon wicks, and tendon interstitial fluid was obtained by centrifugation of pieces of tendon. Spontaneous lymph flow rates averaged 70 nl.min-1.g skin-1. Protein concentrations and colloid osmotic pressure of sampled fluids differed significantly. Tail lymph had the highest protein concentration relative to plasma [lymph-to-plasma ratio 0.71 ± 0.03 (SE) n = 10], followed by wick fluid (0.62 ± 0.02, n = 9), with tendon fluid lowest (0.50 ± 0.03, n = 10). Albumin and immunolgobulin G (IgG) concentrations in samples of tail skin and tendon were assayed by rocket immunoelectrophoresis. Comparison of their distribution volumes at lymph or tendon fluid concentrations, respectively, with interstitial fluid volumes measured as 2-h 51Cr-ethylenediaminetetraacetic acid space minus 5-min 125I-albumin space indicated that 50-60% of the interstitial volume in these tissues is not available for distribution of albumin or IgG. Low lymph flow and high interstitial protein content or rat tail indicate a slow turnover of interstitial protein. This suggests that interstitial washout of protein plays a role in limiting edema only after a sustained or chronic increase in fluid filtration.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021269226&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0021269226
VL - 16
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1
ER -