Protein kinase C-dependent activation of NF-κB in enterocytes is independent of IκB degradation

L. Wilson, Csaba Szabo, A. L. Salzman

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background and Aims: Nuclear translocation of the NF-κB family of transcription factors is a proximal step in the signal transduction of a pleiotropic group of proinflammatory genes. Activation of RelA is under the negative control of IκB, a family of proteins degraded in response to immunologic and oxidant stimuli. The aim of this study was to examine this mechanism of NF-κB activation in intestinal epithelial cells. Methods: DLD- 1 cell monolayers stimulated by interleukin (IL)-1β or phorbol myristate acetate (PMA) were assayed for the nuclear translocation of NF-κB and immunoreactivity of various IκB isoforms that regulate NF-κB1/RelA activation. Results: NF-κB activation triggered by PMA was not associated with the disappearance of immunoreactive IλBα, IκBβ, IκBγ, or IκBε or with the dissociation of intact IκB from RelA. NF-κB activation induced by PMA was blocked by the protein kinase C inhibitor staurosporine but not by the proteasomal inhibitor N-acetyl-leucine leucine norleucinal (ALLN). In contrast, IL-lβ-induced NF-κB activation was associated with the disappearance of IκBα and was inhibited by ALLN but not staurosporine. Conclusions: Our data imply the existence of a novel pathway of NF-κB activation mediated by protein kinase C that does not require proteosomal degradation or the loss of IκB.

Original languageEnglish (US)
Pages (from-to)106-114
Number of pages9
JournalGastroenterology
Volume117
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

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Enterocytes
Leucine
Protein Kinase C
Tetradecanoylphorbol Acetate
Staurosporine
Protein C Inhibitor
Interleukins
Protein Kinase Inhibitors
Interleukin-1
Oxidants
Signal Transduction
Protein Isoforms
Transcription Factors
Epithelial Cells
Genes
Proteins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Protein kinase C-dependent activation of NF-κB in enterocytes is independent of IκB degradation. / Wilson, L.; Szabo, Csaba; Salzman, A. L.

In: Gastroenterology, Vol. 117, No. 1, 1999, p. 106-114.

Research output: Contribution to journalArticle

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