Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors

Jhumku D. Kohtz, Sharon F. Jamison, Cindy L. Will, Ping Zuo, Reinhard Lührmann, Mariano A. Garcia-Blanco, James L. Manley

Research output: Contribution to journalArticle

502 Scopus citations

Abstract

Exactly how specific splice sites are recognized during the processing of complex precursor messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are involved, but are not sufficient by themselves to define splice sites. Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA. This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalNature
Volume368
Issue number6467
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

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    Kohtz, J. D., Jamison, S. F., Will, C. L., Zuo, P., Lührmann, R., Garcia-Blanco, M. A., & Manley, J. L. (1994). Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors. Nature, 368(6467), 119-124. https://doi.org/10.1038/368119a0