Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors

Jhumku D. Kohtz, Sharon F. Jamison, Cindy L. Will, Ping Zuo, Reinhard Lührmann, Mariano Garcia-Blanco, James L. Manley

Research output: Contribution to journalArticle

486 Citations (Scopus)

Abstract

Exactly how specific splice sites are recognized during the processing of complex precursor messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are involved, but are not sufficient by themselves to define splice sites. Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA. This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalNature
Volume368
Issue number6467
StatePublished - Mar 10 1994
Externally publishedYes

Fingerprint

RNA Splice Sites
RNA Precursors
U1 Small Nuclear Ribonucleoproteins
Protein Splicing
Small Nuclear Ribonucleoproteins
Proteins
Alternative Splicing
Serine
Arginine
RNA Splicing Factors

ASJC Scopus subject areas

  • General

Cite this

Kohtz, J. D., Jamison, S. F., Will, C. L., Zuo, P., Lührmann, R., Garcia-Blanco, M., & Manley, J. L. (1994). Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. Nature, 368(6467), 119-124.

Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. / Kohtz, Jhumku D.; Jamison, Sharon F.; Will, Cindy L.; Zuo, Ping; Lührmann, Reinhard; Garcia-Blanco, Mariano; Manley, James L.

In: Nature, Vol. 368, No. 6467, 10.03.1994, p. 119-124.

Research output: Contribution to journalArticle

Kohtz, JD, Jamison, SF, Will, CL, Zuo, P, Lührmann, R, Garcia-Blanco, M & Manley, JL 1994, 'Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors', Nature, vol. 368, no. 6467, pp. 119-124.
Kohtz JD, Jamison SF, Will CL, Zuo P, Lührmann R, Garcia-Blanco M et al. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. Nature. 1994 Mar 10;368(6467):119-124.
Kohtz, Jhumku D. ; Jamison, Sharon F. ; Will, Cindy L. ; Zuo, Ping ; Lührmann, Reinhard ; Garcia-Blanco, Mariano ; Manley, James L. / Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. In: Nature. 1994 ; Vol. 368, No. 6467. pp. 119-124.
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