Protein restriction during pregnancy induces hypertension and impairs endothelium-dependent vascular function in adult female offspring

Kunju Sathishkumar, Rebekah Elkins, Uma Yallampalli, Chandra Yallampalli

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Intrauterine undernutrition plays a role in the development of adult hypertension. Most studies are done in male offspring to delineate the mechanisms whereby blood pressure may be raised; however, the vascular mechanisms involved in female offspring are unclear. Female offspring of pregnant Sprague-Dawley rats fed either a control (C; 18%) or a low-protein (LP; 6%) diet during pregnancy were used. Birth weight and later growth were markedly lower in LP than in C offspring. LP offspring exhibited impaired estrous cyclicity with increased mean arterial pressure. Hypotensive response to acetylcholine (ACh) and the hypertensive response to phenylephrine (PE) were greater in LP than in C rats. N-nitro-L-arginine methyl ester (L-NAME) induced greater hypertensive responses in C than in LP rats. Endothelium-intact mesenteric arteries from LP offspring exhibited increased contractile responses to PE and reduced vasodilation in response to ACh. In endothelium-denuded arteries, relaxation responses to sodium nitroprusside were similar in both groups. Basal and ACh-induced increase in vascular nitrite/nitrate production was lower in LP than in C offspring. L-NAME or 1H-1,2,4-oxadiazolo-4,3- quinoxalin-1-one inhibited ACh relaxations and enhanced PE contractions in C offspring, but had minimal effect in LP rats. The decreasedNO-mediated vascular response might explainthe increased vascular contraction and arterial pressure infemale offspring with low birth weight.

    Original languageEnglish (US)
    Pages (from-to)229-239
    Number of pages11
    JournalJournal of Vascular Research
    Volume46
    Issue number3
    DOIs
    StatePublished - Apr 2009

    Fingerprint

    Vascular Endothelium
    Adult Children
    Acetylcholine
    Blood Vessels
    Phenylephrine
    Hypertension
    Pregnancy
    NG-Nitroarginine Methyl Ester
    Endothelium
    Arterial Pressure
    Proteins
    Quinoxalines
    Mesenteric Arteries
    Low Birth Weight Infant
    Nitroprusside
    Periodicity
    Nitrites
    Birth Weight
    Vasodilation
    Malnutrition

    Keywords

    • Constriction
    • Endothelium
    • Fetal programming
    • Hypertension
    • Mean arterial blood pressure
    • Nitric oxide
    • Pregnancy
    • Relaxation
    • Vascular smooth muscle

    ASJC Scopus subject areas

    • Physiology
    • Cardiology and Cardiovascular Medicine

    Cite this

    Protein restriction during pregnancy induces hypertension and impairs endothelium-dependent vascular function in adult female offspring. / Sathishkumar, Kunju; Elkins, Rebekah; Yallampalli, Uma; Yallampalli, Chandra.

    In: Journal of Vascular Research, Vol. 46, No. 3, 04.2009, p. 229-239.

    Research output: Contribution to journalArticle

    Sathishkumar, Kunju ; Elkins, Rebekah ; Yallampalli, Uma ; Yallampalli, Chandra. / Protein restriction during pregnancy induces hypertension and impairs endothelium-dependent vascular function in adult female offspring. In: Journal of Vascular Research. 2009 ; Vol. 46, No. 3. pp. 229-239.
    @article{8b2a6240a0324ae2b25444449fea0a8e,
    title = "Protein restriction during pregnancy induces hypertension and impairs endothelium-dependent vascular function in adult female offspring",
    abstract = "Intrauterine undernutrition plays a role in the development of adult hypertension. Most studies are done in male offspring to delineate the mechanisms whereby blood pressure may be raised; however, the vascular mechanisms involved in female offspring are unclear. Female offspring of pregnant Sprague-Dawley rats fed either a control (C; 18{\%}) or a low-protein (LP; 6{\%}) diet during pregnancy were used. Birth weight and later growth were markedly lower in LP than in C offspring. LP offspring exhibited impaired estrous cyclicity with increased mean arterial pressure. Hypotensive response to acetylcholine (ACh) and the hypertensive response to phenylephrine (PE) were greater in LP than in C rats. N-nitro-L-arginine methyl ester (L-NAME) induced greater hypertensive responses in C than in LP rats. Endothelium-intact mesenteric arteries from LP offspring exhibited increased contractile responses to PE and reduced vasodilation in response to ACh. In endothelium-denuded arteries, relaxation responses to sodium nitroprusside were similar in both groups. Basal and ACh-induced increase in vascular nitrite/nitrate production was lower in LP than in C offspring. L-NAME or 1H-1,2,4-oxadiazolo-4,3- quinoxalin-1-one inhibited ACh relaxations and enhanced PE contractions in C offspring, but had minimal effect in LP rats. The decreasedNO-mediated vascular response might explainthe increased vascular contraction and arterial pressure infemale offspring with low birth weight.",
    keywords = "Constriction, Endothelium, Fetal programming, Hypertension, Mean arterial blood pressure, Nitric oxide, Pregnancy, Relaxation, Vascular smooth muscle",
    author = "Kunju Sathishkumar and Rebekah Elkins and Uma Yallampalli and Chandra Yallampalli",
    year = "2009",
    month = "4",
    doi = "10.1159/000166390",
    language = "English (US)",
    volume = "46",
    pages = "229--239",
    journal = "Journal of Vascular Research",
    issn = "1018-1172",
    publisher = "S. Karger AG",
    number = "3",

    }

    TY - JOUR

    T1 - Protein restriction during pregnancy induces hypertension and impairs endothelium-dependent vascular function in adult female offspring

    AU - Sathishkumar, Kunju

    AU - Elkins, Rebekah

    AU - Yallampalli, Uma

    AU - Yallampalli, Chandra

    PY - 2009/4

    Y1 - 2009/4

    N2 - Intrauterine undernutrition plays a role in the development of adult hypertension. Most studies are done in male offspring to delineate the mechanisms whereby blood pressure may be raised; however, the vascular mechanisms involved in female offspring are unclear. Female offspring of pregnant Sprague-Dawley rats fed either a control (C; 18%) or a low-protein (LP; 6%) diet during pregnancy were used. Birth weight and later growth were markedly lower in LP than in C offspring. LP offspring exhibited impaired estrous cyclicity with increased mean arterial pressure. Hypotensive response to acetylcholine (ACh) and the hypertensive response to phenylephrine (PE) were greater in LP than in C rats. N-nitro-L-arginine methyl ester (L-NAME) induced greater hypertensive responses in C than in LP rats. Endothelium-intact mesenteric arteries from LP offspring exhibited increased contractile responses to PE and reduced vasodilation in response to ACh. In endothelium-denuded arteries, relaxation responses to sodium nitroprusside were similar in both groups. Basal and ACh-induced increase in vascular nitrite/nitrate production was lower in LP than in C offspring. L-NAME or 1H-1,2,4-oxadiazolo-4,3- quinoxalin-1-one inhibited ACh relaxations and enhanced PE contractions in C offspring, but had minimal effect in LP rats. The decreasedNO-mediated vascular response might explainthe increased vascular contraction and arterial pressure infemale offspring with low birth weight.

    AB - Intrauterine undernutrition plays a role in the development of adult hypertension. Most studies are done in male offspring to delineate the mechanisms whereby blood pressure may be raised; however, the vascular mechanisms involved in female offspring are unclear. Female offspring of pregnant Sprague-Dawley rats fed either a control (C; 18%) or a low-protein (LP; 6%) diet during pregnancy were used. Birth weight and later growth were markedly lower in LP than in C offspring. LP offspring exhibited impaired estrous cyclicity with increased mean arterial pressure. Hypotensive response to acetylcholine (ACh) and the hypertensive response to phenylephrine (PE) were greater in LP than in C rats. N-nitro-L-arginine methyl ester (L-NAME) induced greater hypertensive responses in C than in LP rats. Endothelium-intact mesenteric arteries from LP offspring exhibited increased contractile responses to PE and reduced vasodilation in response to ACh. In endothelium-denuded arteries, relaxation responses to sodium nitroprusside were similar in both groups. Basal and ACh-induced increase in vascular nitrite/nitrate production was lower in LP than in C offspring. L-NAME or 1H-1,2,4-oxadiazolo-4,3- quinoxalin-1-one inhibited ACh relaxations and enhanced PE contractions in C offspring, but had minimal effect in LP rats. The decreasedNO-mediated vascular response might explainthe increased vascular contraction and arterial pressure infemale offspring with low birth weight.

    KW - Constriction

    KW - Endothelium

    KW - Fetal programming

    KW - Hypertension

    KW - Mean arterial blood pressure

    KW - Nitric oxide

    KW - Pregnancy

    KW - Relaxation

    KW - Vascular smooth muscle

    UR - http://www.scopus.com/inward/record.url?scp=54849408073&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=54849408073&partnerID=8YFLogxK

    U2 - 10.1159/000166390

    DO - 10.1159/000166390

    M3 - Article

    VL - 46

    SP - 229

    EP - 239

    JO - Journal of Vascular Research

    JF - Journal of Vascular Research

    SN - 1018-1172

    IS - 3

    ER -