Protein synthesis is required for cholera toxin-induced stimulation of arachidonic acid metabolism

Johnny Peterson, James C. Reitmeyer, Christopher A. Jackson, Ghulam Ansari

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The molecular events in the mechanism of action of cholera toxin were analyzed using Chinese hamster ovary (CHO) cells. Cholera toxin stimulated both 3′,5′-cyclic adenosine monophosphate (cAMP) synthesis and arachidonic acid metabolism in these cells. The turnover of phospholipid by cholera toxin-induced stimulation of phospholipase activity evoked the synthesis of PGE2 and other prostaglandins. Cholera toxin-induced release of both [3H]arachidonic acid and PGE2 was blocked by addition of either cycloheximide or actinomycin D. In contrast, accumulation of cAMP in cholera toxin-treated CHO cells was unaffected by adding these drugs. Further, dibutyryl cAMP or forskolin caused [3H]arachidonic acid release, which also was blocked by cycloheximide and actinomycin D. We concluded that the sequence of molecular events in cholera toxin-treated CHO cells first involved activation of adenylate cyclase, which caused an increase in cAMP. In turn, cAMP promoted transcription of mRNA that encoded either a specific phospholipase or a phospholipase-activating protein. The emerging arachadonic acid metabolites (e.g., PGE2 and PGF) might be important mediators of cholera toxin's stimulatory effects on vascular permeability and smooth muscle contraction in the intestine during cholera.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
JournalBBA - Molecular Cell Research
Volume1092
Issue number1
DOIs
StatePublished - Mar 19 1991

Fingerprint

Cholera Toxin
Arachidonic Acid
Cyclic AMP
Phospholipases
Cricetulus
Dinoprostone
Proteins
Ovary
Dactinomycin
Cycloheximide
Dinoprost
Cholera
Capillary Permeability
Colforsin
Muscle Contraction
Vascular Smooth Muscle
Adenylyl Cyclases
Prostaglandins
Intestines
Phospholipids

Keywords

  • (Mouse Chinese hamster ovary cell)
  • Arachidonic acid metabolism
  • Cholera toxin
  • cyclic AMP synthesis
  • Prostaglandin synthesis
  • Protein synthesis

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Protein synthesis is required for cholera toxin-induced stimulation of arachidonic acid metabolism. / Peterson, Johnny; Reitmeyer, James C.; Jackson, Christopher A.; Ansari, Ghulam.

In: BBA - Molecular Cell Research, Vol. 1092, No. 1, 19.03.1991, p. 79-84.

Research output: Contribution to journalArticle

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