Proteinase-activated receptor-2 activation induces uterine contractility in term pregnant rats that is not dependent on mast cell activation and cyclo-oxygenase products

Holger Maul, Egle Bytautiene, Yuri Vedernikov, Robert E. Garfield, George Saade, Dinesh Shah

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVES: This study was undertaken to evaluate the effect of proteinase-activated receptor-2 (PAR-2) activation on the contractility of uterine tissues from term pregnant rats and the role of mast cells and prostaglandins in such an effect. STUDY DESIGN: Uterine rings from pregnant (day 20-21) Sprague-Dawley rats were used for isometric tension recording in organ chamber experiments (Krebs solution, 5% carbon dioxide in air, 37°C, pH ∼7.4). Responses to the PAR-2 activating peptide SLIGRL (serine-leucine-isoleucine-glycine-arginine-leucine), and to the inactive reverse peptide LRGILS (leucine-arginine-glycine-isoleucine-leucine-serine) were determined after pretreatments with compound 48/80, cromolyn, S[+]-chlorpheniramine maleate, cimetidine, combinations of histamine (H) receptor antagonists with cromolyn or ibuprofen and compared with vehicle. RESULTS SLIGRL significantly augmented contractility of uterine tissues, and this response was not inhibited by compound 48/80, cromolyn, and ibuprofen, as well as by H1- and H2-receptor antagonists, alone or in combination with cromolyn. CONCLUSION: PAR-2 activation augments uterine contractility in tissues obtained from term pregnant rats, and this effect is independent of mast cell activation or cyclo-oxygenase pathway products.

Original languageEnglish (US)
Pages (from-to)1498-1503
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume188
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

PAR-2 Receptor
Prostaglandin-Endoperoxide Synthases
Cromolyn Sodium
Mast Cells
Leucine
Isoleucine
Glycine
Serine
p-Methoxy-N-methylphenethylamine
Ibuprofen
Arginine
Chlorpheniramine
Histamine Receptors
Histamine H1 Receptors
Histamine H2 Receptors
Cimetidine
Carbon Dioxide
Prostaglandins
Sprague Dawley Rats
Air

Keywords

  • Histamine
  • Mast cells
  • Myometrium
  • PAR-2
  • Rat

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Proteinase-activated receptor-2 activation induces uterine contractility in term pregnant rats that is not dependent on mast cell activation and cyclo-oxygenase products. / Maul, Holger; Bytautiene, Egle; Vedernikov, Yuri; Garfield, Robert E.; Saade, George; Shah, Dinesh.

In: American Journal of Obstetrics and Gynecology, Vol. 188, No. 6, 01.06.2003, p. 1498-1503.

Research output: Contribution to journalArticle

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