Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates

Gabriele Neumann, Thomas Geisbert, Hideki Ebihara, Joan B. Geisbert, Kathleen M. Daddario-DiCaprio, Heinz Feldmann, Yoshihiro Kawaoka

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Enveloped viruses often require cleavage of a surface glycoprotein by a cellular endoprotease such as furin for infectivity and virulence. Previously, we showed that Ebola virus glycoprotein does not require the furin cleavage motif for virus replication in cell culture. Here, we show that there are no appreciable differences in disease progression, hematology, serum biochemistry, virus titers, or lethality in nonhuman primates infected with an Ebola virus lacking the furin recognition sequence compared to those infected with wild-type virus. We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates.

Original languageEnglish (US)
Pages (from-to)2995-2998
Number of pages4
JournalJournal of Virology
Volume81
Issue number6
DOIs
StatePublished - Mar 2007
Externally publishedYes

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Ebolavirus
Furin
Virus Replication
virus replication
Primates
glycoproteins
Glycoproteins
Virulence
virulence
pathogenicity
Viruses
Subtilisin
viruses
subtilisin
Membrane Glycoproteins
Hematology
hematology
viral load
Viral Load
disease course

ASJC Scopus subject areas

  • Immunology

Cite this

Neumann, G., Geisbert, T., Ebihara, H., Geisbert, J. B., Daddario-DiCaprio, K. M., Feldmann, H., & Kawaoka, Y. (2007). Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates. Journal of Virology, 81(6), 2995-2998. https://doi.org/10.1128/JVI.02486-06

Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates. / Neumann, Gabriele; Geisbert, Thomas; Ebihara, Hideki; Geisbert, Joan B.; Daddario-DiCaprio, Kathleen M.; Feldmann, Heinz; Kawaoka, Yoshihiro.

In: Journal of Virology, Vol. 81, No. 6, 03.2007, p. 2995-2998.

Research output: Contribution to journalArticle

Neumann, G, Geisbert, T, Ebihara, H, Geisbert, JB, Daddario-DiCaprio, KM, Feldmann, H & Kawaoka, Y 2007, 'Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates', Journal of Virology, vol. 81, no. 6, pp. 2995-2998. https://doi.org/10.1128/JVI.02486-06
Neumann, Gabriele ; Geisbert, Thomas ; Ebihara, Hideki ; Geisbert, Joan B. ; Daddario-DiCaprio, Kathleen M. ; Feldmann, Heinz ; Kawaoka, Yoshihiro. / Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates. In: Journal of Virology. 2007 ; Vol. 81, No. 6. pp. 2995-2998.
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