Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Pia Davidsson, Ann Westman-Brinkmalm, Carol L. Nilsson, Maria Lindbjer, Linda Paulson, Niels Andreasen, Magnus Sjögren, Kaj Blennow

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, β-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalNeuroReport
Volume13
Issue number5
StatePublished - Apr 16 2002
Externally publishedYes

Fingerprint

Cerebrospinal Fluid Proteins
Proteome
Alzheimer Disease
Apolipoprotein E2
Retinol-Binding Proteins
Proteins
Prealbumin
Apolipoproteins
Ubiquitin
Electrophoresis
Mass Spectrometry
Protein Isoforms
Gels
Staining and Labeling
Technology

Keywords

  • Alzheimer's disease
  • Apolipoproteins
  • Cerebrospinal fluid
  • Mass spectrometry
  • Proteomics
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Davidsson, P., Westman-Brinkmalm, A., Nilsson, C. L., Lindbjer, M., Paulson, L., Andreasen, N., ... Blennow, K. (2002). Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients. NeuroReport, 13(5), 611-615.

Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients. / Davidsson, Pia; Westman-Brinkmalm, Ann; Nilsson, Carol L.; Lindbjer, Maria; Paulson, Linda; Andreasen, Niels; Sjögren, Magnus; Blennow, Kaj.

In: NeuroReport, Vol. 13, No. 5, 16.04.2002, p. 611-615.

Research output: Contribution to journalArticle

Davidsson, P, Westman-Brinkmalm, A, Nilsson, CL, Lindbjer, M, Paulson, L, Andreasen, N, Sjögren, M & Blennow, K 2002, 'Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients', NeuroReport, vol. 13, no. 5, pp. 611-615.
Davidsson P, Westman-Brinkmalm A, Nilsson CL, Lindbjer M, Paulson L, Andreasen N et al. Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients. NeuroReport. 2002 Apr 16;13(5):611-615.
Davidsson, Pia ; Westman-Brinkmalm, Ann ; Nilsson, Carol L. ; Lindbjer, Maria ; Paulson, Linda ; Andreasen, Niels ; Sjögren, Magnus ; Blennow, Kaj. / Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients. In: NeuroReport. 2002 ; Vol. 13, No. 5. pp. 611-615.
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