Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Pia Davidsson; Ann Westman-Brinkmalm; Carol L. Nilsson; Maria Lindbjer; Linda Paulson; Niels Andreasen; Magnus Sjögren; Kaj Blennow

doi:10.1097/00001756-200204160-00015

Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Pia Davidsson, Ann Westman-Brinkmalm, Carol L. Nilsson, Maria Lindbjer, Linda Paulson, Niels Andreasen, Magnus Sjögren, Kaj Blennow

Research output: Contribution to journal › Article › peer-review

190 Scopus citations

Abstract

By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, β-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

Original language	English (US)
Pages (from-to)	611-615
Number of pages	5
Journal	NeuroReport
Volume	13
Issue number	5
DOIs	https://doi.org/10.1097/00001756-200204160-00015
State	Published - Apr 16 2002
Externally published	Yes

Keywords

Alzheimer's disease
Apolipoproteins
Cerebrospinal fluid
Mass spectrometry
Proteomics
Two-dimensional electrophoresis

ASJC Scopus subject areas

General Neuroscience

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10.1097/00001756-200204160-00015

Cite this

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AU - Davidsson, Pia

AU - Westman-Brinkmalm, Ann

AU - Nilsson, Carol L.

AU - Lindbjer, Maria

AU - Paulson, Linda

AU - Andreasen, Niels

AU - Sjögren, Magnus

AU - Blennow, Kaj

PY - 2002/4/16

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AB - By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, β-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

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KW - Apolipoproteins

KW - Cerebrospinal fluid

KW - Mass spectrometry

KW - Proteomics

KW - Two-dimensional electrophoresis

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Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Abstract

Keywords

ASJC Scopus subject areas

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