Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Pia Davidsson, Ann Westman-Brinkmalm, Carol L. Nilsson, Maria Lindbjer, Linda Paulson, Niels Andreasen, Magnus Sjögren, Kaj Blennow

Research output: Contribution to journalArticlepeer-review

179 Scopus citations

Abstract

By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, β-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalNeuroReport
Volume13
Issue number5
DOIs
StatePublished - Apr 16 2002

Keywords

  • Alzheimer's disease
  • Apolipoproteins
  • Cerebrospinal fluid
  • Mass spectrometry
  • Proteomics
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Neuroscience(all)

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